大豆抗癌肽的分离纯化及分子量分布  

Purification and distribution of molecular weight of soybean anti-cancer peptides

作　　者：葛锡娟[1] 赵城彬[1] 杨丽丽[1] 吴非[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2012年第3期99-101,105,共4页Science and Technology of Food Industry

基　　金：黑龙江省攻关项目(GA09B401-2);哈尔滨市科技创新人才基金项目(2008RFQXN016)

摘　　要：为确定具有抗癌活性大豆多肽的分子量,对大豆抗癌肽粗提物进行超滤,采用MTT法(四噻唑蓝比色法)对得到的不同组分超滤液进行抗癌活性检测,初步确定分子量在5000～10000u范围的组分具有最高的抑癌活性,大豆抗癌肽的IC50为0.47mg/mL。用葡聚糖SephadexG-75凝胶柱对该组分进行进一步分离,收集各洗脱组分并进行抗癌活性研究。结果显示:组分A4具有最佳的抗癌活性,其IC50为0.37mg/mL。根据分子量标准曲线,得到其平均相对分子质量为6723u。To determine the molecular weight of soybean peptides with anti-cancer activity,the extraction of soybean anti-cancer peptides was ultrafiltrated.MTT（thiazole blue four） assay was used to detect the anti-cancer activity of different components obtained by ultrafiltration.Components of soybean anti-cancer peptides with molecular weight ranging from 5000 to 10000u had the highest anti-cancer activity,and the IC50 of soy anti-cancer peptides was 0.47mg/mL.Gel Filtration Chromatography（Sephadex G-75） was applied to further separate and collect all of fractions in order to research the anti-cancer activity.The results showed that：A4 components had the best anti-cancer activity,and its IC50 was 0.37mg/mL.According to standard curve of molecular weight,average molecular weight of A4 was about 6723u.

关 键 词：大豆多肽 抗癌活性 凝胶过滤 相对分子量 

分 类 号：TS201.23[轻工技术与工程—食品科学]