分光光度法测定葡萄酒中尿素含量  

Spectrophotometric determination of urea level in wine

作　　者：梁新红[1] 杨大光[1] 孙俊良[1] 郭祖峰[1] 唐玉[1] 

机构地区：[1]河南科技学院食品学院,河南新乡453003

出　　处：《食品工业科技》2012年第4期76-79,共4页Science and Technology of Food Industry

基　　金：河南省教育厅自然科学研究资助计划项目(2010A550004);河南科技学院博士基金资助项目(08001)

摘　　要：目的:建立了分光光度法定量检测葡萄酒中尿素含量的方法。方法:采用强阳性离子交换树脂柱对葡萄酒中尿素进行分离,依据费伦反应原理,采用分光光度法测定洗脱液中尿素含量。结果:建立方法的最佳参数为丁二酮单肟最佳添加量6.0g/L,氨基硫脲最佳添加量0.10g/L,反应中煮沸时间为5min,用分光光度计在530nm波长下测定吸光度。方法的最低检出限0.5mg/L,相对标准偏差为3.1%～4.4%,回收率为92.8%～103.1%。结论:此方法简便快速、准确可靠,适合用于葡萄酒样品中尿素含量的定量检测。Objective：A method was described for colorimetric determination of urea in wine.Method：Urea in wine samples was extrated with the cation-exchange resin,and then urea in the elute was measured based on Fearon reaction with spectrophotometer.Result：The results showed that the optimal conditions as follows：the concentration of diacetyl monoxime 6.0g/L,thiosemicarbazide 0.10g/L,and the mixture boiled at 100℃ for 5min,and measure absorbance at 530nm.The determination limit of the method of wine samples was 0.5mg/L,and the RSD were 3.1% ～ 4.4%,and recoveries were 92.8% ～ 103.1%.Conclusion：The method presented in this paper was accurate and reliable,and it was suitable for determination of urea in wine.

关 键 词：分光光度法 葡萄酒 尿素 费伦试剂 

分 类 号：TS207.3[轻工技术与工程—食品科学]