北虫草液态发酵天然培养基的优化研究  

Optimization of natural medium for fermentation of Cordyceps militaris

作　　者：解春艳[1] 顾振新[2] 顾颖娟[2] 曾波[2] 张亮[2] 

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000 [2]南京农业大学农业部农畜产品加工与质量控制重点开放实验室,江苏南京210095

出　　处：《食品工业科技》2012年第4期223-226,共4页Science and Technology of Food Industry

基　　金：廊坊师范学院博士基金项目(LSZB201003);廊坊市科学技术研究与发展计划项目(2011012001)

摘　　要：对北虫草液态发酵天然培养基进行优化研究,经比较确定适宜液态发酵的北虫草菌株及最佳氮源,之后采用混料设计对发芽糙米浆、麦芽汁和豆粕汁三种原料的组成配比进行优化,确定适宜北虫草液态发酵的最佳培养基。研究表明:北虫草CM-3菌株适宜液态发酵,且该菌在以豆粕为氮源时生长最好;发芽糙米浆、麦芽汁和豆粕汁(可溶性固形物含量分别为6%)混料比为55:5:40时,CM-3的菌丝干重达最高(17.0g/L)。The optimization of natural medium for fermentation of Cordyceps militaris was investigated.Firstly,the optimal stain of Cordyceps militaris and optimal nitrogen were screened.And then,the mixture ratio of the fermentation medium making up of germinated brown rice paste,beerwort and soybean meal for Cordyceps militaris were optimized by mixture design.The soluble solids contents of the three materials were 6%,respectively.Results indicated that CM-3 and soybean were the optimal fungus and optimal nitrogen for the submerged fermentation,respectively.And the highest yield of mycelia（17.0g/L） was obtained at a ratio of 55：5：40.

关 键 词：北虫草 菌丝体 天然培养基 混料设计 液态发酵 

分 类 号：TS201.3[轻工技术与工程—食品科学]