聚酰胺吸附-HPLC测定饮料和糖果中的20种水溶性色素  

Determination of 20 Water-soluble Pigments in Beverages and Candy by Polyamide

作　　者：顾宇翔[1] 葛宇[1] 印杰[1] 虞成华[1] 陆志芸[1] 刘孟鲲[1] 马晓飞[1] 

机构地区：[1]上海市质量监督检验技术研究院/国家食品质量监督检验中心(上海),上海200233

出　　处：《食品与发酵工业》2012年第1期161-164,共4页Food and Fermentation Industries

基　　金：国家质量监督检验检疫总局科研项目(2009QK106)

摘　　要：建立了聚酰胺吸附-HPLC测定饮料和糖果中的20种水溶性色素的方法。样品用水提取,经聚酰胺粉末吸附-洗脱后,以Eclipse XDB C18柱分析(250 mm×4.6 mm,5μm),采用0.02 mol/L乙酸铵-甲醇-乙腈梯度洗脱,检测波长分别选取450、520和620 nm。该方法线性范围2.00～50.0μg/mL,重现性相对标准偏差(n=6)为0.51%～7.8%,回收率71.4%～109.2%,检出限0.03～0.3 mg/kg。Research developed a polyamide absorption-HPLC method to determine 20 kinds of water-soluble pigments in beverages and candy.Samples were extracted with water,then absorbed-desorbed by polyamide,finally analyzed by Eclipse XDB C18 column（250 mm ×4.6 mm,5 μm）.The mobile phase was： 0.02 mol/L ammonium acetate – methanol-acetonitrile;wavelength was 450nm,520 nm and 620nm,respectively.A good linear relationship was obtained from 2.00 μg/mL to 50.0 μg/mL.The R.S.D.of samples reproducibility（n=6） was 0.51%~7.8%;the recovery of the method was in the range of 71.4%~109.2%;the limit of detection was 0.03~0.3 mg/kg.The method was accurate and reproducible,it was suitable for analysis of several water-soluble pigments in beverages and candy simultaneously.

关 键 词：聚酰胺 HPLC 饮料 糖果 色素 测定 

分 类 号：TS202.3[轻工技术与工程—食品科学]