膨大处理对红地球葡萄酚类物质及抗氧化活性的影响  

Effect of Enlargement Treatment on Phenolic Content and Antioxidant Activities of Red Globe Grape

作　　者：刘金串[1] 孟江飞[1] 郭志君[1] 耿万刚[1] 乔玲[1] 张昂[1] 张振文[1,2] 房玉林[1,2] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100

出　　处：《食品科学》2012年第5期7-12,共6页Food Science

基　　金：国家现代农业(葡萄)产业技术体系项目(nycytx-30-2p-04);国家林业局"948"项目(2009-04-09);西安市科技创新支撑计划项目(NC10003);农业部杨凌苗木繁殖基地项目

摘　　要：以红地球葡萄为研究对象,对葡萄进行膨大处理后,定期采样,测定处理组和对照组的总酚、总黄酮、总黄烷醇以及铜离子还原力、铁氰化钾还原力、DPPH自由基清除率、金属螯合力、羟自由基(.OH)清除率,用DPS软件对处理组和对照组之间的差异及酚类物质与抗氧化活性之间的相关性进行分析。结果表明:膨大处理可增加红地球葡萄酚类物质含量并增强其抗氧化活性;膨大处理对红地球葡萄总酚、总黄酮、总黄烷醇、铜离子还原力、铁氰化钾还原力的影响较大,对DPPH自由基清除率、金属螯合力、.OH清除率的影响较小。铜离子还原力、铁氰化钾还原力与酚类物质(总酚、总黄酮、总黄烷醇)含量均呈极显著正相关;金属螯合力、DPPH自由基清除率与酚类物质含量均无显著相关性,.OH清除率与酚类物质含量呈极显著负相关。铜离子还原力与铁氰化钾还原力呈极显著正相关;与DPPH自由基清除率呈显著正相关。铜离子还原力与.OH清除率、铁氰化钾还原力与.OH清除率呈极显著负相关。The effect of enlargement treatment on total phenols and antioxidant activities of red globe grapes were determined in this study. The phenolic compounds and antioxidant activities were investigated during the berry growth and maturation after enlargement treatments. The difference between the treatment and the control, the correlation coefficients between phenolic compositions and antioxidant capacity, as well as the correlation coefficients among different methods for quantifying antioxi- dant capacity were analyzed based on DPS. The results showed that the content of total phenols and antioxidant activities of red globe grapes could be increased by enlargement treatments. The effect of enlargement treatment on total polyphenols, total flavonoids, total flavanols, cupric reducing power, potassium ferricyanide reducing power were higher than on DPPH radical scavenging capacity, ferrous ion chelating capacity and hydroxyl radical scavenging activity. The phenolic compounds had a significant positive correlation with cupric reducing power and potassium ferricyanide reducing power, but a significant negative correlation with hydroxyl radical scavenging activity. Both DPPH radical scavenging capacity and ferrous ion chelating capacity had no positive correlation with phenolic compounds. A significant positive correlation between cupric reducing power and potassium ferricyanide reducing power, and positive correlation between cupric reducing power and DPPH radical scavenging capacity were observed. The potassium ferricyanide reducing power and cupric reducing power had a significant negative correlation with hydroxyl radical scavenging activity.

关 键 词：鲜食葡萄 膨大处理 酚类物质 抗氧化活性 

分 类 号：S663.1[农业科学—果树学]