旱生植物内生细菌的分离及耐旱菌株的筛选鉴定  

Isolation and Identification of Drought-tolerant Bacteria from Xerophtes

作　　者：张彦涛[1] 生吉萍[1,2] 葛佳[1] 矫玉翠[1] 王正荣[1] 程凡升[1] 申琳[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国人民大学农业与农村发展学院,北京100872

出　　处：《食品科学》2012年第5期124-128,共5页Food Science

基　　金：国家公益性行业(农业)科研专项(200803033)

摘　　要：为筛选耐旱内生细菌,并对其进行鉴定,以典型耐旱植物为材料,分离纯化出142株内生细菌。采用液体培养基中添加聚乙二醇6000降低渗透势的方法分析内生细菌的耐旱情况。从中筛选出1株能够耐受质量浓度为60g/100mL聚乙二醇6000的内生细菌PB14。对该菌株进行形态学观察,生理生化和16S rDNA序列鉴定,判定菌株PB14为蜡样芽孢杆菌(Bacillus cereus)。Drought-tolerant bacteria were isolated and identified in this paper. One hundred and forty two bacterial strains were isolated from the typical drought-tolerant plants. Through adding polyglycol 6000 （PEG6000） to the fluid nutrient medium for decreasing osmotic potential, the drought tolerance of endophytic bacteria were analyzed. One of these strains, PB 14, has the capability of drought resistance up to 60 g/100 mL PEG6000. This strain was identified as Bacillus cereus based on morphological and biochemical experiments as well as 16S rDNA sequence analysis.

关 键 词：耐旱 内生细菌 鉴定 蜡样芽孢杆菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]