Protex 6L直接酶解红豆粉提取红豆肽的研究  

Study on enzymolysis extraction of red bean peptide from red bean powder by Protex 6L

作　　者：刘琪[1] 江连洲[1,2] 李扬[1,2] 王梅[1] 王胜男[1] 齐宝坤[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]国家大豆工程技术研究中心,黑龙江哈尔滨150030

出　　处：《食品工业科技》2012年第7期191-194,210,共5页Science and Technology of Food Industry

基　　金：黑龙江省攻关项目(GA09B401-6);农业部现代农业产业技术体系建设项目(nycytx-004)

摘　　要：采用Protex 6L直接酶解红豆粉的方法提取红豆肽,通过响应曲面分析得出Protex 6L蛋白酶提取红豆蛋白的最佳条件:pH为9.4,加酶量为3.14%,温度为60℃,时间为3.5h,料液比为1∶6.9(g/mL)。并采用凝胶层析过滤色谱分析了不同水解时间水解物分子量的变化,发现红豆蛋白中存在一些不容易被Protex 6L酶解的成分,红豆粉的Protex 6L酶解过程是一个不均匀的过程。The red bean peptide was extracted from red bean by the aqueous enzymatic method using Protex 6L.The results showed that optimum conditions with response surface analysis were：enzyme amount 3.14%,hydrolysis temperature 60℃,enzymolysis time 3.5h,the ratio of solid to liquid 1∶6.9,pH9.4.The molecular masses of the hydrolysates were determined with Sephadex G-75 gel filtration chromatography.It showed that red bean contained some stable components which were difficult to hydrolyze with Protex 6L and the process of red bean hydrolysis was disproportional.

关 键 词：Protex 6L 酶解 红豆肽 响应面法 

分 类 号：TS201.2[轻工技术与工程—食品科学]