宁海白枇杷花中酚类物质鉴定及含量测定  

Identification and Determination of Phenols in Loquat Flower by HPLC-PAD-MS

作　　者：闫永芳[1] 过尘杰[1] 孙钧[1] 孟天真[1] 赵春江[1] 叶兴乾[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310058

出　　处：《中国食品学报》2012年第4期202-207,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：教育部创新工程重大项目培育资金(707034);浙江省重大科技专项(2008C02005-2)

摘　　要：运用HPLC-PAD-MS技术对宁海白枇杷花中酚类化合物进行定性及定量分析,共检测到6种主要酚类化合物,4种被鉴定。其中,异槲皮苷含量最高,达到1.536mg/g;绿原酸0.5550mg/g;槲皮苷0.5836mg/g;槲皮素含量较低,0.2294mg/g。通过回收率试验、系统稳定性试验及日间精密度试验对该方法进行验证,结果显示各物质的回收率在90.3%～101.2%之间,方法准确性好;各标品峰高及峰面积的变异系数均小于1%(n=3),系统稳定性较好;各标品保留时间的变异系数均小于1%,日间精密度良好。本分析方法具有快速、灵敏、准确的优点,适用于枇杷花中酚类物质的定性及定量分析。Phenols in NingHai white loquat flower were identified and determined using a HPLC-PAD-MS method in the research. Six compounds were detected and four of them were identified. The identified phenols were chlorogenic acid （0.5550mg/g）, quercetin-3-o-glucoside （1.536mg/g）, quercitrin （0.5836mg/g） and quercetin （0.2294mg/g）. The recovery test was between 90.3% and 101.2%, and the CV of stability and inter-day repeatability were lower than 1%. The results showed that the established method was a fast, effective and accurate method to analyze phenols in loquat flower.

关 键 词：酚类物质 枇杷花 HPLC-PAD-MS 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]