利用16S rRNA序列鉴定分离自芽菜中的芽孢杆菌  

Identification of Bacillus strains isolated from Yacai by 16S rRNA gene sequencing

作　　者：张良[1] 吴华昌[1] 邓静[1] 李萍萍[1] 肖辰[1] 沈芳[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000

出　　处：《中国酿造》2012年第5期82-84,共3页China Brewing

基　　金：四川省科技厅应用基础项目(2010JY0081)

摘　　要：从宜宾芽菜中分离优势菌群,选取4株芽孢杆菌,分别为B1、B2、B3、B4。对4株菌的16S rRNA基因经PCR扩增测序,将测序结果同该属内菌株的16S rRNA序列作多序列比较,并建立芽孢杆菌属的系统发育树。结合细菌形态学生理生化特性鉴定结果,结果表明菌株B1、B3为枯草芽孢杆菌,菌株B2为解淀粉芽孢杆菌,菌株B4为乙酰微小杆菌。Four dominant Bacillus strains named B1,B2,B3 and B4 were isolated from Yacai （a kind of Yibin pickles in China）.The 16S rRNA genes of these strains were amplified in vitro and sequenced.Then a phylogenetic tree was constructed by multiple alignments of their sequences with other 16S rRNA gene sequences of Bacillus.According to 16S rRNA gene analysis combined with morphological,physiological and biochemical characteristics,B1 and B3 were identified asBacillus subtilis,B2 wasBacillus amyloliquefaciens and B4 wasExiguobacterium acetylicum.

关 键 词：芽菜 16S RRNA 芽孢杆菌 鉴定 

分 类 号：TS201.1[轻工技术与工程—食品科学] Q939.1[轻工技术与工程—食品科学与工程]