基于双向电泳和质谱联用技术的水牛乳源酪蛋白的研究  

Analysis of water buffalo milk in south China by two-dimensional electrophoresis and mass spectrometry combined technology

作　　者：向明霞[1] 王丽娜[1] 李子超[1] 成希飞[1] 徐明芳[1] 

机构地区：[1]暨南大学生命科学技术学院生物工程系,广东广州510632

出　　处：《食品工业科技》2012年第12期188-190,200,共4页Science and Technology of Food Industry

摘　　要：利用双向电泳和基质辅助激光解吸电离飞行时间质谱(MALDI-MS)联用技术对水牛乳酪蛋白与其他乳源蛋白差异性进行了研究。根据Image Master 2D Platinum图像分析软件对不同乳源酪蛋白的双向电泳(2-DE)图谱进行蛋白斑点的匹配分析,获得21个存在于水牛奶中,主要分布在低丰度蛋白区的差异蛋白点,经质谱分析,得到4个属于水牛奶酪蛋白的主要组分,另外发现两个与水牛奶中的蛋白有较高同源性的新组分。Two-dimensional electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry（MALDI-MS）combined technology was used to analysis the differences between in water buffalo milk in south China and other milk proteins.21 differential proteins which mainly distributed in areas of low abundance existed only in water buffalo milk were identified by comparing two-dimensional electrophoresis（2-DE）map of casein spots matching analysis with software of image master 2D platinum.Four casein fractions mainly could belong to water buffalo casein and two new fractions which were high homology components with water buffalo casein were determined with the further analysis by mass spectrometry（MS）.

关 键 词：水牛奶 酪蛋白 双向电泳 质谱 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]