食品过敏原荞麦的实时荧光PCR检测  

Detection of buckwheat in food by real-time PCR

作　　者：张舒亚[1] 李妮[2] 刘金华[3] 高琴[1] 陈颖[4] 郭云霞[2] 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]上海海洋大学,上海201306 [3]吉林出入境检验检疫局,长春130062 [4]中国检验检疫科学研究院,北京100123

出　　处：《中国卫生检验杂志》2012年第6期1237-1239,共3页Chinese Journal of Health Laboratory Technology

基　　金：上海技术标准专项(10DZ0504300)

摘　　要：目的:荞麦是日本法规要求强制性标识的食品过敏原,我国需要建立出口日本食品中过敏原荞麦成分的标准方法。方法:参考现有文献,采用实时PCR方法建立食品过敏原荞麦成分的检测方法。结果:研究表明建立的检测方法具有特异性,灵敏度高,检测限为1 mg/kg。结论:本方法适用于食品中过敏原荞麦成分检测。Objective：To develop a method for detection of buckwheat in food exported to Japan based on that buckwheat was the mandatory-signed food allergen in Japan.Methods：A real-time PCR method was established for detection of buckwheat in food.Results：The method was specific for buckwheat,and the limit of detection was 1 mg/kg.Conclusion：The PCR procedure is suitable for the detection of buckwheat in food.

关 键 词：食品过敏原 荞麦 实时荧光PCR 

分 类 号：R155.52[医药卫生—营养与食品卫生学]