云南茶树资源儿茶素和没食子酸的差异性分析  

Difference Analysis on Catechins and Gallic Acid Contents of Tea Resources from Yunnan Province

作　　者：刘本英[1] 宋维希[1] 马玲[1] 段志芬[1] 蒋会兵[1] 孙雪梅[1] 黄玫[1] 矣兵[1] 汪云刚[1] 王平盛[1] 

机构地区：[1]云南省农业科学院茶叶研究所,云南勐海666201

出　　处：《西南农业学报》2012年第3期864-869,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家现代农业(茶叶)产业技术体系建设专项资金(nycytx-23);国家自然科学基金(31160175);云南省农科院茶叶所所列项目(2009A0937);农业部保种项目(NB2011-2130135-01);云南省科技创新人才计划(2011CI068)

摘　　要：以勐海国家种质茶树资源圃中的121个云南茶树资源为材料,采用高效液相色谱法(HPLC)测定茶叶中的没食子酸(GA)、儿茶素(C)、表儿茶素(EC)、表儿茶素没食子酸酯(ECG)、表没食子酸儿茶素(EGC)及表没食子儿茶素没食子酸酯(EGCG)的含量。结果表明,GA含量变幅为0.210%～1.902%,平均0.834%,在茶树中含量较低。C含量变幅为0.069%～8.865%,平均1.916%;EC含量变幅为0.126%～2.865%,平均1.112%;EGC含量变幅为0.00%～3.709%,平均0.954%;ECG的含量变幅为0.739%～8.957%,平均4.063%;EGCG含量变幅为0.819%～11.77%,平均5.939%;儿茶素总量含量为6.354%～22.654%,平均14.042%。资源间儿茶素差异较大,说明云南茶树资源具有丰富的生物多样性。同时,筛选出3份高EGCG含量(≥10%)的特异茶树资源,为高EGCG茶树品种选育提供了重要材料。By using high performance liquid chromatography （HPLC）, the gallic acid （GA）, eatechins （ C ）, epicateehin （ EC ）, epigalloeat-echin（EGC） , epicatechin gallate （ECG） and epitllocatechin gallate （EGCG） contents of 121 tea germplasms farom the China National C, ermplasm Tea Repositories （CNGTR） at the Tea Research Institute of Yunnan Academy of Agricultural Sciences （TRIYAAS） were meas- ured. The results showed that the content of GA ranged from 0.210 % to 1. 902 %, with an average of O. 834 %, explaining relatively low GA content among tea germplasms; the content of C ranged from 0. 069 % to 8.865 %, with an average of 1. 916 % ; the content of EC ranged from 0. t26 % to 2. 865 %, with an average of 1.112 % ; the content of EGC ranged from 0.00 to 3. 709 %, with an average of 0. 954 % ; the content of ECG ranged from 0.739 % to 8. 957 %, with an average of 4. 063 % ; the content of EGCG ranged from 0.819 % to 11.77 %, with an average of 5. 939 % ; and the conteut of total C ranged from 6.354 % to 22. 654 %, with an average of 14.042 %. There was relatively big difference of eatechin contents among different tea resources, indicating that there was plentiful biodiversity of Yun- nan tea germplasms. At the same time, three tea germplasms with high epigallocatechin gal]ate content （ ≥ 10 % ） was selected preliminari- ly, which would provide important materials for breeding tea cultivars with high EGCG content in the future.

关 键 词：云南 茶树资源 没食子酸 儿茶素 差异性 

分 类 号：S571.1[农业科学—茶叶生产加工]