体内外消化高直链抗性水稻淀粉的波谱特性  

Spectroscopic Properties of in vivo and in vitro Digestive Resistant Starch from High-Amylose Transgenic Rice

作　　者：满建民[1] 周兴华[2] 董英[2] 刘巧泉[1] 韦存虚[1] 

机构地区：[1]扬州大学教育部植物功能基因组学重点实验室,江苏省作物遗传生理重点实验室,江苏扬州225009 [2]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品科学》2012年第13期26-30,共5页Food Science

基　　金：农业部转基因生物新品种培育科技重大专项(2009ZX-08011-003B);国家自然科学基金面上项目(31071342);江苏省作物学优势学科资助项目

摘　　要：以基因工程培育出来的高直链抗性淀粉水稻(TRS)为材料,利用粉末X射线衍射、傅里叶变换红外光谱分析技术,研究体内外消化TRS淀粉的波谱特性。结果表明:不被消化的抗性淀粉直链淀粉含量显著升高;TRS淀粉为C型晶体淀粉,由A型和B型晶体淀粉组成,其中A型晶体比B型晶体消化降解快,B型晶体具有抗消化降解的作用;TRS淀粉的无定形区域易被消化降解。In this study, the spectroscopic properties of in vivo and in vitro digestive resistant starches from a high-amylose transgenic rice line （TRS） established through antisense RNA inhibition of starch branching enzymes were investigated by X-ray powder diffraction and Fourier transform infrared spectroscopy. The results showed that the amylose contents of non-digestive TRS resistant starch significantly increased. TRS starch was C-type crystalline starch, a mixture of A- and B-type crystalline starches. The degradation of the A-type crystalline starch was faster than that of B-type starch, and the B-type crystalline starch showed a high resistance to digestion. The amorphous starch of starch granule was easy to be degraded. These data could be useful for various applications of high-amylose crop in the food and nonfood industry.

关 键 词：水稻 高直链淀粉 体内消化 体外消化 波谱特性 

分 类 号：Q946.3[生物学—植物学] TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]