HS-SPME与GC-MS联用分析不同季节艾纳香叶香气成分  

Headspace Solid-Phase Microextraction Coupled with GC-MS for Analysis of Aromatic Components in Leaves of Blumea balsamifera (L.) DC. in Different Seasons

作　　者：王远辉[1] 王洪新[1,2] 田洪芸[1] 马朝阳[1] 陈涛[1] 邹纯礼 王兴 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [3]贵州艾源生态药业开发有限公司,贵州罗甸550100

出　　处：《食品科学》2012年第14期166-170,共5页Food Science

基　　金：科技型中小企业技术创新基金项目(11C26215205838);贵州省科技型中小企业技术创新基金项目(黔科合字[2011]5002号)

摘　　要：采用顶空固相微萃取法和气质联用技术分别对秋季和冬季艾纳香叶香气成分进行分析。结果表明:秋季样品香气成分为49种(91.37%),其中萜类化合物为43种(81%);冬季样品香气成分为47种(95.87%),其中萜类化合物为37种(88.48%)。秋季样品相对含量前5位的依次为(E)-石竹烯(24.88%),L-龙脑(17.86%),(+)-γ-古芸烯(12.27%),花椒素(7.83%),(E)-罗勒烯(3.6%)。冬季样品相对含量前五位的依次为L-龙脑(47.3%),樟脑(13.64%),(E)-石竹烯(7.41%),(+)-γ-古芸烯(3.9%),β-石竹烯环氧化物(2.75%)。两种样品相同的香气成分有31种,因此两样品的主体香气相似,即樟脑香、胡椒香和药香,但两样品中相同组分相对含量差异很大。此外秋季样品还含有18种独有组分,冬季样品含有16种,因此不同季节生产的艾纳香精油香气不同,各有特色。The aroma components in leaves of Blumea balsamifera （L.） DC. harvested in fall and winter were extracted by headspace solid-phase micro-extraction （HS-SPME） and analyzed by gas chromatography-mass spectrometry （GC-MS）. The results showed that 49 aroma components were found in fall leaves （91.37%）, including 43 terpenoids （81%）; 47 aroma components were found in winter leaves, including 37 terpenoids （88.48%）. The top five components were （E）-caryophyllene （24.88%）, 1-borneol （17.86%）, （＋）- y-gurjunene （12.27%）, xanthoxylin （7.83%） and （E）-ocimene （3.6%） in fall samples, and 1-bomeol （47.3%）, camphor （13.64%）, （E）-caryophyllene （7.41%）, （＋）-y-gurjunene （3.9%） and β-caryophyllene epoxide （2.75%） in winter samples. Fall and winter samples contained 31 aroma components in common and therefore had similar aroma characteristics, namely camphor, pepper and herbal aroma. However, a significant difference in the relative contents of the aroma components common to samples harvested in both seasons was found. Eighteen and 16 components were exclusive to winter and fall samples, respectively. In conclusion, the volatile oil components in Blumea balsamifera （L.） DC. vary seasonally.

关 键 词：固相微萃取 气质联用 艾纳香叶 香气成分 

分 类 号：O657.63[理学—分析化学]