ε-聚赖氨酸与纳他霉素复配剂抑菌性能的研究  

Synergistic antibacterial action of ε-polylysine in combination with natamycin

作　　者：宋庆超[1] 李秀雯[1] 王洪翠[1] 钟成[1] 贾士儒[1] 

机构地区：[1]天津科技大学工业微生物教育部重点实验室,天津300457

出　　处：《中国酿造》2012年第7期80-83,共4页China Brewing

基　　金："十二五"农村领域国家科技计划课题(2011BAD23B05-3)

摘　　要：研究了ε-聚赖氨酸(ε-PL)与纳他霉素复配剂的抑菌活性。以典型菌株大肠杆菌、伤寒沙门氏菌、金黄色葡萄球菌、枯草芽孢杆菌、酿酒酵母和热带假丝酵母为研究对象,采用二倍稀释法确定ε-PL和纳他霉素的最小抑菌浓度,96孔板法确定ε-PL与纳他霉素对酵母菌最佳复配抑菌浓度,通过测定胞内物质渗漏来反映酵母细胞膜的完整性。结果表明,二者抑制酿酒酵母的最佳复配浓度为ε-PL 100μg/mL、纳他霉素4μg/mL,抑制热带假丝酵母的最佳复配浓度为ε-PL 400μg/mL、纳他霉素2μg/mL,且两种复配剂均能抑制大部分细菌。通过测定抑菌剂作用于酿酒酵母在260nm、280nm的吸光度以及电导率的变化,可知复配剂对酿酒酵母细胞膜的破坏性最强。总之,ε-PL与纳他霉素复配使用,能够减少用量,拓宽抑菌范围,能更好的抑制食品中腐败微生物的生长。The antibacterial activity of the complex of ε-PL and natamycin is studied.Typical strains,including E.coli,typhoid salmonella,staphylococcus aureus,bacillus subtilis,wine yeast and tropical candida are used as the research object.We use 2-fold dilution method to determine the minimum inhibitory concentration of ε-PL and natamycin,96 orifice plate to determine the optimal yeast inhibitory concentration of ε-PL and natamycin and reflect the integrity of the yeast cell membrane by measuring the leakage of intracellular substances.The results show that optimal compound concentration of the Saccharomyces cerevisiae was 100μg/ml（ε-PL） and 4μg/ml（natamycin）,the concentration of Candida tropicalis was 400μg/ml（ε-PL） and 2μg/ml（natamycin）,and two kinds of complex could inhibit most bacteria.Through detecting the change of antibacterial agents in the role of s.cerevisiae in 260nm,280nm spectrophotometry and electric conductivity,it can be seen that the yeast cell membrane is damaged most seriously by the complex.In one word,the complex of ε-PL and natamycin can reduce dosage,broaden the scope of bacteriostasis,and be better able to restrain the growth of microorganism.

关 键 词：Ε-聚赖氨酸 纳他霉素 复配剂 协同作用 

分 类 号：TS202.3[轻工技术与工程—食品科学]