新疆酸马奶中高产胞外多糖乳酸菌筛选鉴定及培养条件优化研究  

Study on Screening and Identification of a Lactic Acid Bacterium with High EPS-producing Capacity and Optimization of Culture Conditions in Koumiss in Xinjiang

作　　者：唐血梅[1] 李海英[1] 赵芳[1] 姚新奎[1] 董婷[1] 车驰[1] 祝春梅[1] 刘婷婷[1] 曾亚琦[1] 韩煜茹[1] 曹丽萍[1] 王建文[1] 刘志安[1] 孟军[1] 李智军[1] 

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2012年第8期1540-1545,共6页Xinjiang Agricultural Sciences

基　　金：国家农业部公益性行业科研专项"马驴产业技术研究与试验示范项目"(201003075)

摘　　要：【目的】从新疆伊犁牧民自制酸马奶中筛选出一株高产胞外多糖的乳酸菌,并对该菌产胞外多糖的培养条件进行优化,从而得到更多的胞外多糖,为乳酸菌产胞外多糖的培养条件提供理论依据。【方法】应用苯酚硫酸法筛选出产胞外多糖量高的乳酸菌,通过生理生化和糖发酵实验对产糖量高的乳酸菌进行鉴定;在培养条件的优化试验中,采用单次单因子法,探索初始pH值、培养时间、碳源及氮源等对乳酸菌产胞外多糖量的影响。【结论】鉴定出产胞外多糖量高的乳酸菌为干酪乳杆菌,并且确定干酪乳杆菌产胞外多糖的最佳合成条件为葡萄糖2%,蛋白胨1.5%,发酵时间28 h,初始pH值6.5,此条件下所合成胞外多糖量为121.6 mg/L。[ Objective ] A lactic acid bacteria with high EPS - producing capacity was screened from kou- miss in Ili, Xinjiang, and the culture conditions of the lactobacillus was optimized in order to gain more EPS and provide theoretical basis for the culture conditions of lactobacillus with EPS - producing capacity. [ Meth- od] Production of EPS was measured by Phenol - H2SO4 method. This lactic acid bacteria with high EPS - producing capacity was identified by physiological biochemical characters and sugar fermentation tests. In or- der to study the effect of single factor on the EPS biosynthesis capacity, optimization of carbon and nitrogen source in medium and culture conditions such as time, pH for the lactic acid bacteria were carried out in the paper. [ Conclusion ] The results showed that this lactic acid bacteria was identified as Laetobacillus casei. Glucose （ 2% ） and peptone （ 1.5% ） was favorable carbon and nitrogen source for this lactohaeillus casei to produce EPS. The optimal culture conditions for producing EPS were ： fermented for 28 h, initial pH 6.5. The maximum biosynthesis amount of EPS was 121.6 mg/L.

关 键 词：胞外多糖 乳酸菌 酸马奶 

分 类 号：S879.2[农业科学—畜牧兽医] S188.4