植物乳杆菌ST-Ⅲ胆盐水解酶的表达及其酶活力分析  

Expression and Activity Analysis of Bile Salt Hydrolases from Lactobacillus plantarum ST-Ⅲ

作　　者：任婧 姚晶[1,2] 

机构地区：[1]光明乳业研究院乳业生物技术国家重点实验室,上海200436 [2]上海海洋大学食品学院,上海201306

出　　处：《食品科学》2012年第17期165-168,共4页Food Science

基　　金：国家"973"计划项目(2010CB735705);上海市科学技术委员会科研计划项目(09DZ2251400)

摘　　要：以植物乳杆菌(Lactobacillus plantarum)ST-Ⅲ4种胆盐水解酶(BSHs)的编码序列(bsh 1～4),将其克隆至表达载体pET-28b(+)上,在原核系统进行表达,并对其酶活力进行测定,结果发现4种BSHs的酶活力分别为29.00、20.49、24.90、21.13U/mL。同时BSH1比其他3种BSHs表现出更高的水解能力。In vertebrates, bile salt hydrolysis plays an essential role in fat metabolism. Bile salts are synthesized in the liver. In the small intestine, glycine or taurine are de-conjugated from bile salts by the enzyme bile salt hydrolase （BSH, EC 3.5.1.24） from intestinal microbes, which reduces the serum cholesterol level. In this study, four predicted bile salt hydrolase （bsh） genes from Lactobacillus plantarum ST-III were cloned into pET-28b（＋） vector and expressed in Escherichia coli. The hydrolysis activity of these enzymes was 29.00, 20.49, 24.90 U/mL and 21.13 U/mL, respectively.

关 键 词：植物乳杆菌 胆盐水解酶 表达 酶活力 

分 类 号：Q939.117[生物学—微生物学]