生物法合成尿苷二磷酸葡萄糖的研究进展  

Research Advances in Biosynthesis of UDPG

作　　者：陈圣[1] 李艳[1] 刘欢[1] 严明[1] 许琳[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009

出　　处：《中国生物工程杂志》2012年第9期125-130,共6页China Biotechnology

基　　金：江苏省高校自然科学研究(10KJB530004);国家自然科学基金(21106068);江苏省自然科学基金(BK2011801);教育部博士点基金(20113221120002)资助项目

摘　　要：尿苷二磷酸葡萄糖(UDPG)是一种重要的糖类物质合成前体。生物法合成具有低成本、无污染和高立体选择性等传统化学法不具备的优势。利用纯酶催化的生物法以基于Leloir途径改进的一锅法、蔗糖合酶催化的两步法以及糖合成反应可逆催化等产UDPG,实现了UDPG的高产。全细胞催化法利用稳定的胞内酶系产UDPG,胞内生成的UDPG作为底物直接参与产物的催化合成,可行性高且成本更低。综述了酶法和全细胞催化法合成UDPG这两种最主要生物法的研究进展。UDPG is an important nucleotide diphosphate monosaccharide which serves as a biogenetic precursor for a range of sugars. Compared with traditional chemical synthesis of UDPG, Biosynthesis is inexpensive pollution-free and high Stereospecific. Pure enzymatic strategies involving modified Leloir one-pot enzymatic system, two-step Sucrase synthase catalysis and reversible catalysis of sugar synthetic reaction achieved a high production of UDPG. Whole cell catalysis utilized the stable endoenzymes for UDPG biosynthesis and intracellular UDPG could be directly used to synthetize the products in cells, which seemed to be viable and low cost. In this paper, the research advances of enzymatic and whole cell catalysis were reviewed.

关 键 词：尿苷二磷酸葡萄糖 生物法 酶法 全细胞催化 

分 类 号：Q55[生物学—生物化学]