北大西洋瓜参岩藻聚糖硫酸酯分离纯化及结构分析  

Purification and Characterization of Fucoidan Extracted from Cucumaria frondosa

作　　者：于龙[1] 薛长湖[1] 宿玮[1] 王彦超[1] 张翠玉[1] 常耀光[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2012年第19期83-87,共5页Food Science

基　　金：国家海洋局海洋公益性行业科研专项(201105029);国家国际科技合作项目(2010DFA31330);国家自然科学基金项目(30972284);中央高校基本科研业务费专项

摘　　要：目的:从北大西洋瓜参(Cucumaria frondosa)体壁中提取纯化得到一种北大西洋瓜参岩藻聚糖硫酸酯(CF-FUC),并对其结构特征进行研究。方法:利用阴离子交换层析和凝胶过滤层析纯化出CF-FUC组分,以高效凝胶过滤色谱法测定分子质量,以离子色谱法测定硫酸根含量,以柱前衍生高效液相色谱法测定单糖组成,并通过核磁共振技术推测其精细结构。结果:CF-FUC的分子质量为360kD,硫酸根含量为(29.8±3.2)%,单糖组成为岩藻糖、半乳糖、氨基葡萄糖、氨基半乳糖物质的量比为1:0.22:0.07:0.07,岩藻糖存在两位硫酸酯化α型吡喃岩藻糖及非硫酸酯化α型吡喃岩藻糖两种形式。结论:CF-FUC结构有别于其他海参岩藻聚糖硫酸酯(SC-FUC)。In this study, fucoidan from Cucumariafrondosa body wall was purified and structurally characterized. A fraction named as CF-FUC was obtained by anion exchange chromatography and gel filtration chromatography. The fraction was analyzed for molecular weight by high-performance gel filtration chromatography, sulfate content by ion chromatography, monosaccharide composition by pre-column derivitization high-performance liquid chromatography, and fine structural characteristics by nuclear magnetic resonance. The molecular weight of CF-FUC was 360 kD with a sulfate content of （29.8 ± 3.2）%, and its monosaccharide composition included fucose, galactose, glucosamine and galactosamine at a ratio of 1：0.22：0.07：0.07. Moreover, NMR analysis revealed that the fucoidan was mainly composed of a-L-pFuc-2（OSO3） and α-L-pFuc. In conclusion, the structure of Cucumariafrondosa fucoidan is novel and different from that reported previously for sea cucumber fucoidan.

关 键 词：海参 北大西洋瓜参 海参多糖 岩藻聚糖硫酸酯 结构 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]