法夫酵母产虾青素培养基及培养条件的优化  

Optimization of Medium and Culture Conditions for Astaxanthin Production by Phaffia Rhodozyma

作　　者：洪清林[1] 肖安风[1,2] 倪辉[1,2,3] 李利君[1] 蔡慧农[1,2,3] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]福建省高校食品微生物与酶工程技术研究中心,福建厦门361021 [3]厦门市食品与生物工程技术研究中心,福建厦门361021

出　　处：《中国食品学报》2012年第10期131-139,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(20702019);福建省产学研重大专项项目(2010N5009);福建省自然科学基金项目(2011J01224)

摘　　要：采用单因素实验对高产虾青素的法夫酵母(Phaffia rhodozymaJMU-MVP14)的培养基及培养条件进行优化,确定最佳培养基组成是:葡萄糖30g/L,酵母膏6g/L,(NH4)2SO41.015g/L(C/N=65),MgSO4·7H2O1g/L,KH2PO413.6g/L(C/P=10),CaCl20.2g/L。最佳培养条件是:初始pH7.0,接种量5%,装液量30mL,培养温度20℃。在此条件下,于7L发酵罐、进行发酵培养,其生物量和虾青素含量分别为13.13g/L和67.07mg/L,与初始培养条件相比分别提高了85.8%和15.8%。Astaxanthin production was investigated by single factor in Phaffia rhodozyma JMU-MVP14 fermentation. The optimal culture medium for astaxanthin production was glucose 30 g/L, yeast extract 6 g/L, （NH4）2SO4 1.015 g/L, MgSO4·7H2O 1 g/L, KH2PO4 13.6 g/L, CaCl2 0.2 g/L, and the optimal culture conditions were initial pH 7.0, inoculum size 5%, volume of liquid 30 mL and culture temperature 20 ℃. Using the optimal condition in 7 L bioreactor, the biomass and astaxanthin production increased 85.8% and 15.8%, respectively. The biomass reached to 13.13 g/L, and the astaxanthin production reached to 67.07 mg/L.

关 键 词：法夫酵母 虾青素 优化 

分 类 号：TQ929[轻工技术与工程—发酵工程]