乙醇冷浴辅助酶法提取大豆油工艺研究  

Aqueous enzymatic extraction of soybean oil assisted with ethanol in cold bath

作　　者：刘琪[1] 江连洲[1,2] 李杨[1,2] 王胜男[1] 王梅[1] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]国家大豆工程技术研究中心,哈尔滨150030

出　　处：《中国油脂》2012年第11期4-7,共4页China Oils and Fats

基　　金：黑龙江省攻关项目(GA09B401-6);农业部现代农业产业技术体系建设项目(nycytx-004)

摘　　要：采用向大豆酶解脱磷液(游离油、乳状液和水解液)中加入乙醇冷浴破乳,应用响应面优化分析方法对乙醇冷浴破乳条件进行优化。优化范围为:冷浴温度-30～-40℃,乙醇与脱磷酶解液体积比1∶(0.75～1.25),乙醇体积分数70%～90%,冷浴时间25～45 min。所建立的回归模型拟合良好,R2=0.952 1。通过回归模型分析,得出最优响应结果为:冷浴温度-34℃,乙醇与脱磷酶解液体积比1∶1.06,乙醇体积分数81%,冷浴时间36 min。在最优条件下,大豆油得率达到(91.25±0.61)%。Ethanol in cold bath assisted technology was employed to demulsify the emulsions of the free oil, emulsions and hydrolysate in soybean dephosphorization enzymatic hydrolysate. The response surface method- ology （RSM） was applied to optimize the reaction conditions. The optimal ranges were as follows：cold bath temperature -30 - -40℃, volume ratio of ethanol to dephosphofization enzymatic hydrolysate 1：0. 75 - 1：1.25 ,ethanol concentration 70% -90%, and cold bath time 25 -45 min. The established regression model was fitted well with R2 of 0. 952 1. The optimal conditions were ： cold bath temperature - 34 ℃, vol- ume ratio of ethanol to dephosphorization enzymatic hydrolysate 1：1.06 , ethanol concentration 81%, cold bath time 36 min. Under the optimal conditions, soybean oil yield was（91.25 ±0.61 ）%.

关 键 词：乙醇冷浴 水酶法 破乳 大豆油 乳状液 

分 类 号：TS224[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ028[轻工技术与工程—食品科学与工程]