一株高产胞外多糖米酒乳杆菌的诱变筛选  

Mutagenesis-screening of a high-yield exopolysaccharide of Lactobacillus. Sake

作　　者：白丽娟[1] 季中梅[1] 李向东[2] 

机构地区：[1]辽宁医学院食品科学与工程学院,辽宁锦州121001 [2]光明乳业股份有限公司乳业研究院,乳业生物技术国家重点实验室,上海200436

出　　处：《食品工业科技》2012年第23期195-197,共3页Science and Technology of Food Industry

基　　金：辽宁医学院青年科技启动基金项目(Y2011Z019)

摘　　要：通过对一株高产胞外多糖的米酒乳杆菌BXR-5-3在不同紫外线照射距离和时间、不同浓度亚硝基胍以及紫外线、亚硝基胍复合诱变等条件下分别诱变筛选和多糖含量测定,获得的胞外多糖产量最高诱变菌株的条件为紫外线照射距离为20cm,亚硝基胍的浓度为300μg/mL,处理时间为20min,其多糖的产量为339.0mg/L。It was studied that a strain of Lactobacillus.Sake BXR-5-3 which had high-yield of exopolysaccharides was mutagenized in different distance and time of ultraviolet ray, different concentration of nitrosoguanidine and composite mutagenesis by ultraviolet ray-nitrosoguanidine. It was showed that the best conditions for the composite mutagenesis was the distance of ultraviolet ray for 20cm,the time for 10min and the concentration of nitrosoguanidine for 300μg/mL,the yield of exopolysaccharides was 339.0mg/L.

关 键 词：胞外多糖 米酒乳杆菌 诱变 筛选 

分 类 号：TS201.3[轻工技术与工程—食品科学]