两种弧菌显色培养基在疑似副溶血性弧菌鉴定中的效果比较  

Comparison of Two Kinds of Vibrio Chromogenic Medium for Identification of Presumptive Vibrio parahaemolyticus

作　　者：徐晓可[1] 吴清平[1] 张菊梅[1] 吴葵[1] 张淑红[1] 郭伟鹏[1] 

机构地区：[1]广东省菌种保藏与应用重点实验室、广东省微生物应用新技术公共实验室、广东省华南应用微生物重点实验室一省部共建国家重点实验室培育基地广东省微生物研究所,广东广州510070

出　　处：《现代食品科技》2012年第12期1806-1809,共4页Modern Food Science and Technology

基　　金：广州市科技计划项目(2010U1-E00611)

摘　　要：为了比较环凯和科玛嘉弧菌显色培养基对食品中可疑菌株的初步鉴定作用,本文按国标(GB/T4789.7-2008)程序,以环凯和科玛嘉弧菌显色培养基初步鉴定硫代硫酸盐-柠檬酸盐-胆盐-蔗糖琼脂(TCBS)的副溶血性弧菌可疑菌株,最后用API和MID鉴定证实。在97份食品样品的定性与定量检测中,TCBS疑似菌株有109株,对应样品有31份。环凯和科玛嘉弧菌显色培养基对109株TCBS可疑菌株初步鉴定的结果相同,均检出64株显紫色的可疑菌株,对应样品22份。经API和MID确认,阳性菌株60株,阳性样品为22份,阳性率为22.68%。两种培养基初步鉴定TCBS可疑菌株的敏感性、特异性和符合性均分别为100.00%、91.84%和96.33%。环凯和科玛嘉弧菌显色培养基对TCBS的可疑菌株具有较好的初步鉴定效果。The presumptive Vibrio Parahaemolyticus strains isolated by thiosulfate - citrate - bile salts - sucrose agar （TCBS） from food samples were preliminary identified by HKM and CHROMagar vibrio chromogenic media according to the National Standards （ GB/T4789.7- 2008） and finally were confirmed by the API and MID identification. In qualitative and quantitative detection of 97 food samples, suspect strains appeared in 109/970 TCBS plates, corresponding to 31 samples. 109 suspect strains from different TCBS plates were inoculated with two kinds of chromogenic media. The results showed that there were 64 purple strains, corresponding to 22 samples. 60 strains were identified as V. parahaemolyticus by APL20E among 64 purple strains, corresponding to 22 samples, the positive rate was 22.68%. The sensitivity, specificity, and accordance of two media were 100.00%, 91.84% and 96.33% respectively. HKM and CHROMagar vibrio chromogenic media can be recommended as good choice for preliminary identification of TCBS suspicious strains from foods.

关 键 词：弧菌显色培养基 食品 TCBS可疑菌株 副溶血性弧菌 

分 类 号：TS207.4[轻工技术与工程—食品科学]