葡萄酒乳酸菌种内遗传多样性鉴定  

Intraspecific Genetic Diversity of Lactic Acid Bacteria from Grape Wine

作　　者：但霞[1] 李凯[1] 朱成龙[1] 刘树文[1,2] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100

出　　处：《食品科学》2012年第21期183-186,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2012BAD31B07);中央高校基本科研业务费专项

摘　　要：为了研究葡萄酒乳酸菌从自然启动苹乳发酵的葡萄酒中分离乳酸菌,并对其进行生化鉴定和16S rRNA序列测定,结果显示分离菌种有酒类酒球菌、马里乳杆菌、植物乳杆菌和短乳杆菌4种乳酸菌。同时利用单酶切扩增片段长度多态性(SE-AFLP)对其进行种内基因多样性鉴定,并绘制种内进化树,结果表明SE-AFLP在乳酸菌分类研究中能够达到菌株水平的区分。Lactic acid bacteria isolated from spontaneously Malolactic fermented grape swine were identified by biochemical identification and 16S rRNA sequencing.The results showed that the strains were Oenococcus oeni,Lactobacillus mali,Lactobacillus plantarum and Lactobacillus brevis.Intraspecific genetic diversity of these strains was further analyzed by single-enzyme amplified fragment length polymorphism（SE-AFLP） and phylogenetic tree was constructed by the method of pair group method with arithmetic Mean（UPGMA）.The results showed that SE-AFLP could identify lactic acid bacteria to strain level in lactic acid bacteria classification.

关 键 词：葡萄酒 乳酸菌 SE-AFLP 种内鉴定 

分 类 号：TS261.1[轻工技术与工程—发酵工程]