黑曲霉固态发酵及酶解烟梗条件的研究  

Study on the condition of solid-state fermentation and enzyme hydrolysis of tobacco stem by Aspergillus niger

作　　者：于兴伟[1] 许赣荣[1] 汤朝起[2] 

机构地区：[1]江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡214122 [2]上海烟草集团有限责任公司,上海200082

出　　处：《食品工业科技》2012年第24期195-198,201,共5页Science and Technology of Food Industry

基　　金：上海烟草集团有限责任公司科技项目(SZBCW201000744)

摘　　要：以黑曲霉h1为菌株,以烟梗为发酵培养基进行固态发酵。以发酵后烟梗中果胶残留率为评价指标,优化了三角瓶固态发酵条件,并进行了正交实验。优化的发酵条件为:培养基含水量为料水比1∶1.2,1000mL三角瓶中装料量为40g,接种量为20%,发酵温度为30℃,发酵时间为6d。在此优化条件下进行验证实验,发酵后的烟梗中果胶残留率为27.32%。采用150L厚层通风池式固态发酵罐对烟梗进行发酵并酶解后,残余烟梗中果胶、淀粉及蛋白质等大分子物质残留率分别为35.19%、25.06%及58.74%,说明大部分大分子物质被一定程度的降解并溶于水中。故通过固态发酵的方法可取得较好的降解烟梗中大分子物质的效果。Tobacco stem was used as a major fermentation substrate under solid-state fermentation by Aspergillus niger hl. Fermentation conditions were optimized based on the pectin residual rate in tobacco stem after fermentation and the orthogonal test was carried out. The optimized fermentation showed as follows：the ratio of dry tobacoo stem to water was 1：1.2,40g of tobacco stem was put in a 1000mL triangular flask with inoculum 20%,and the fermentation was carried out at temperature 30~C for 6d. Under above conditions,the verification test was made and the pectin residual rate in tobacco stem after fermentation was 27.32%. Pilot scale experiements were carried out in a 150L thick layer ventilation solid-state fermentation reactor,and after fermentation and enzyme hydrolysis,the residual rates of macromolecular substances,such as pectin,starch or protein in residual tobacco stem were 35.19%,25.06% and 58.74%, respectively,indicating most of macromolecular substances in the tobacco stem could be degraded and dissolved in water. So that,macromolecular substances could be degraded well by solid-state fermentation.

关 键 词：黑曲霉 烟梗 固态发酵 果胶 烟草薄片 

分 类 号：TS444[农业科学—烟草工业]