大豆膳食纤维及其各组分连续分离工艺研究  

The continuous separation technology of dietary fiber and its components from soybean

作　　者：万婕[1] 余海霞[1] 刘成梅[1] 欧阳海峰[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品工业科技》2012年第24期282-285,共4页Science and Technology of Food Industry

基　　金：食品科学与技术国家重点实验室青年基金项目(SKLF-QN-201116)

摘　　要：摘要：对大豆膳食纤维的提取及其各组分的连续分离工艺进行研究。获得酶一化学法提取膳食纤维的最佳工艺条件为：木瓜蛋白酶用量0．012：1（g／g）、碱提液pH12．03、碱提温度68℃，所得膳食纤维样品中总膳食纤维含量为95．57％，其中可溶性膳食纤维17．44％，不溶性膳食纤维78．13％。碱提酸沉法分离半纤维素的最佳工艺条件为：液固比21：1、提取温度35．5℃、提取时间5．3h。该工艺条件下半纤维素的得率为30．15％，纯度为92．34％。酸性次氯酸钠法分离纤维素的最佳反应条件为：次氯酸钠浓度为16％、pH4．0～4．5、提取温度65℃。纤维素样品的得率为37．24％，产品中纤维素含量91．14％。The continuous separation technology of dietary fiber and its components from soybean was studied in this paper. Results showed that the optimum conditions for extracting dietary fiber from soybean by chemical-enzyme method were papain 0.012g/g, pH12.03,temperature 68~C. Under this condition,the contents of TDF,SDF and IDF were 95.57% ,17.44% and 78.13% ,respectively. Hemicellulose was separated by alkali extraction and acid precipitation. The better condition were liquid-solid ratio 21：1,35.5℃ ,and extraction time 5.3h,the yield rate and purity of hemicellulose were 30.15% and 92.34%,respectively. The optimum conditions for separation of cellulose from dietary fiber by acidic sodium hypochlorite were concentration of sodium hypochlorite 16%,pH4.0~4.5,and temperature 65%. The yield rate and content of cellulose were 37.24% and 91.14%, respectively.

关 键 词：膳食纤维 纤维素 半纤维素 连续分离 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]