五种甘薯多糖体外抗氧化活性比较  

In vitro antioxidant activity comparison of sweet potato polysaccharide

作　　者：郭金颖[1] 牟德华[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《粮食与饲料工业》2012年第12期29-31,36,共4页Cereal & Feed Industry

摘　　要：研究通过水提醇沉法得到五种甘薯的多糖,采用柱前衍生高效液相色谱法测定了五种甘薯多糖的单糖组成,并且比较了五种甘薯多糖的抗氧化活性,包括DPPH自由基清除能力、羟自由基清除能力及铁离子还原能力。结果表明:五种甘薯多糖的单糖组成是一致的,都是由葡萄糖、半乳糖和木糖组成,但单糖的比例存在差异。甘薯多糖都具有良好的抗氧化活性,是一种新的抗氧化物质,其中紫薯宁薯2-2对DPPH自由基及羟自由基清除能力最强;而浙薯255的铁离子还原能力最强。在一定程度上说明甘薯多糖的抗氧化活性的作用机理存在差异,这可能与多糖的单糖组成及结构等有着密切的关系。The five kinds of sweet potato polysaccharide were extracted by boiling water, and precipitated by alcohol and u- sing high performance liquid chromatography with pre-column derivatization method to determine the monosaccharide composi- tion and to compare the antioxidant activity, including radical scavenging activity for DPPH and hydroxy and reducing power for iron ion. The results showed that： the five kinds of sweet potato polysaecharide monosaccharide composition were consist- ent, including glucose, glactose and xylose, but the proportio.n of monosaccharides varied; sweet potato polysaccharide had good antioxidant activity, was a new kind of antioxidant substances; the radical scavenging activity for DPPH and hydroxy of purple potato（ning 2-2） was the strongest,while iron ion reducing capacity of Zhejiang potato 255 was the strongest;to a certain extent, the antioxidant activity mechanism of sweet potato polysaccharide existed differences, which may be related to polysac- charides monosaccharide composition and structure.

关 键 词：甘薯多糖 抗氧化性 单糖组成 柱前衍生高效液相色谱 测定 

分 类 号：TS201.2[轻工技术与工程—食品科学]