中高温大曲中黄曲霉的分离鉴定及其安全性初步研究  

Isolation and Identification of Aspergillus flavus from Medium-hightemperature Daqu and Preliminary Research on Its Safety

作　　者：罗惠波[1,2] 杨晓东[1] 李丹宇[1] 叶光斌[1] 王毅[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000 [2]酿酒生物技术及应用四川省重点实验室,四川自贡643000

出　　处：《酿酒科技》2012年第12期37-40,共4页Liquor-Making Science & Technology

基　　金：四川省教育厅重大培育项目(09ZZ015);四川理工学院人才引进项目(2010XJKRL001)

摘　　要：采用传统微生物分离手段从中高温大曲中分离纯化得到黄曲霉菌株NJSYS-15。通过菌落观察、镜检以及分子生物学手段得知其为黄曲霉属;利用AFPA培养基验证其产毒能力,采用酶联免疫法对其产黄曲霉毒素B1进行定量检测,结果表明,其在液态发酵进行到9 d时,黄曲霉毒素B1达到最高值13.6μg/kg。在进行固态模拟发酵时,黄曲霉菌株的接种量加大至10‰,黄曲霉毒素B1含量为3.2μg/kg,仍在国家标准限制以内。A. flavus strain NJSYS-15 was isolated from medium-high-temperature Daqu through traditional microbial isolation method. It was identified as Aspergillus flavus by microscopic observation on the colony and molecular biology methods. Its toxin-producing capacity was verified by AFPA culture medium and the quantity of the produced afiatoxin B1 was detected by Enzyme-linked immunosorbent assays （ELISA）. The results showed that aflatoxin B1 reached to the maximum of 13.6 ug/kg after 9 d liquid fermentation. However, during simulated solid fermentation in the Lab, even as the inoculating quantity of NJSYS-15 increased to 10 ‰, the content of the produced aftatoxin B1 was only 3.2 ug/kg, still within the limits of national standards.

关 键 词：中高温大曲 黄曲霉 ITS 黄曲霉毒素B1 酶联免疫法 

分 类 号：Q93-3[生物学—微生物学] TS261.1[轻工技术与工程—发酵工程]