超声波辅助提取菜用黄麻色素及其抗氧化活性的研究  

Extraction of the pigment from jute with ultrasonic assisted method and its antioxidant activity

作　　者：罗玉芳[1] 祁建民[2] 方艺贞 杨慰恩 陈美霞[3] 

机构地区：[1]福建华南女子职业学院,福建福州350007 [2]福建农林大学生命科学学院,福建福州350002 [3]福建省宁德师范学院,福建宁德352100

出　　处：《福建农林大学学报（自然科学版）》2012年第6期644-649,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家麻类产业技术体系建设(Nycytx-19-E05);福建省麻类种质资源共享创新平台建设(2010N2002)

摘　　要：采用单因素试验分析和正交试验设计,研究超声波辅助提取菜用黄麻色素的工艺条件,用Fenton法测定菜用黄麻色素的抗氧化活性.结果表明:(1)影响色素提取的因素为料液比>超声频率>提取温度>提取时间;(2)提取的最佳条件为使用60∶40(V/V)的0.1%盐酸和95%乙醇作为提取剂,料液比1∶80(g.mL-1),超声频率为中频,提取时间30 min,提取温度为60℃;以此工艺条件提取色素的产率达25.04%;(3)该色素溶液还原力强,具有清除超氧阴离子自由基O2.-和.OH的活性,在色素浓度为0.20 g.L-1时,对超氧阴离子自由基O2.-的清除率为70.11%,对.OH的清除率为83%.Extraction technology of pigment from jute with ultrasonic assisted method was determined with single-factor and orthogo- nal experimental design. Antioxidant activity of extracted pigment was evaluated with Fenton method. The factors affecting extraction rate were ordered as ratios of raw material to solvent 〉 ultrasonic frequency 〉 extraction temperature 〉 extraction time. The optimum conditions were 60：40 （V/V） of 0.1% HCI to 95% alcohol, 1 ： 80 of raw material to solvent, intermediate ultrasonic frequency, ex- traction time 30 rain, extraction temperature 60 ~C. Under these conditions, yield of jute pigment was 25.04%. The pigment had reducing activity and scavenging capacity on 02= and - OH. Scavenging rate on 02= was 70.11%, that on . OH was 83% when the concentration was 0.20 g L-l.

关 键 词：超声波辅助法 正交试验 菜用黄麻 色素 抗氧化活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]