酶法和化学法制备α-亚麻酸甾醇酯的降脂活性研究  

Lipid-lowering Activity of Sterollinolenate Obtained by Enzyme Method and Chemical Method

作　　者：邓乾春[1,2] 许继取[1,2] 郑明明[1,2] 黄凤洪[1,2] 黄庆德[1,2] 杨金娥[1,2] 

机构地区：[1]中国农业科学院油料作物研究所,武汉430062 [2]油料脂质化学与营养湖北省重点实验室,武汉430062

出　　处：《中国食品学报》2012年第12期10-18,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31000777);武汉市青年科技晨光计划(201150431068)

摘　　要：目的:探明由酶法和化学法制备的α-亚麻酸甾醇酯的降脂活性。方法:在对α-亚麻酸甾醇酯组成和品质特性测定的基础上,采用金黄色地鼠高脂模型为研究对象,以市售甾醇酯为对照,研究合成产物对动物血清和肝脏中脂质水平、抗氧化能力以及肝脏LDL受体mRNA表达水平的影响。结果:由酶法和化学法制备的α-亚麻酸甾醇酯纯度在95%以上,共轭二烯值和共轭三烯值未超过2.5mmol/kg和0.6mmol/kg,反式脂肪酸含量小于0.1%。与高脂对照相比,由α-亚麻酸甾醇酯饲喂的金黄地鼠血清中TC,TG,LDL-C和HDL-C以及肝脏中TC,TG水平显著下降,且效果显著优于市售甾醇酯;由α-亚麻酸甾醇酯饲喂的地鼠肝脏中LDL受体mRNA表达水平显著高于高脂对照组和市售甾醇酯组;甾醇酯对机体抗氧化水平均未产生显著影响。结论:酶法和化学法制备的α-亚麻酸甾醇酯组成较为一致,并且均具有显著的降脂活性,但两者没有显著差异,其降脂机制与增加肝脏中LDL受体mRNA表达水平有密切关系。Objective：Lipid-lowering activity of sterollinolenate obtained by enzyme method and chemical method were studied.Method：Composition and quality characteristics of sterollinolenate were determined firstly,effect of sterollinolenate on lipid level,antioxidant activity and LDL-receptor mRNA expressions of hamster fed with high fat diets were studied with sterol ester available on the market as control.Results：Purity of sterollinolenate prepared by enzyme and chemical method were above 95%,conjugated diene,conjugated triene and trans fatty acid in the products were less than 2.5 mmol/kg,0.6 mmol/kg and 0.1% respectively.TC,TG,LDL-C,HDL-C level in serum and TC,TG level in liver of the hamster fed with high fat diet and sterollinolenate decreased significantly compared with the control,the same result was obtained for LDL-receptor mRNA expressions in liver.Sterollinolenate had no effect on antioxidant activity of hamster.Conclusion：Sterollinolenates obtained by enzyme and chemical method with the same compositions possessed significant lipid-lowering activity,the mechanism was related with the increase of LDL-receptor mRNA expressions in liver.There was no significant difference in lipid-lowering activity between sterollinolenates prepared by different technologies.

关 键 词：α-亚麻酸甾醇酯 品质特性 金黄地鼠 降脂活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]