短乳杆菌BS6 GlnR介导的氮代谢调控研究  

GlnR-Mediated Regulation for Nitrogen Metabolism in Lactobacillus Brevis BS6

作　　者：江湖[1,2] 刘志文[1] 李昆太[1] 郑小梅[2] 伍宁丰[2] 徐波[1] 

机构地区：[1]江西农业大学生物科学与工程学院,南昌330045 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《中国食品学报》2012年第12期19-24,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31060208);江西省教育厅科研基金项目(GJJ11407);南昌市科技局科研基金项目〔(洪科发计字(2011)158-52〕

摘　　要：短乳杆菌是乳酸杆菌属的重要菌种,被广泛应用于食品和医药行业。全局性转录因子GlnR普遍存在于各种细菌中,可以调控多个基因的表达。本研究从短乳杆菌(Lactobacillus brevis)BS6基因组扩增得到GlnR全长编码基因及包含GlnR盒的glnRA,amtB-glnK,glnPQ启动子序列,并运用细菌单杂交的方法证明GlnR在细菌体内可与上述3个操纵子的启动子结合,从而初步验证全局性转录因子GlnR在短乳杆菌氮代谢调控中的重要作用。As an important strain of lactobacillus,Lactobacillus brevis has been widely used in food and medicine industries.The global transcriptional regulator GlnR which is widely found in a variety of bacteria,regulates the transcription of several genes.The full-length sequence of Lactobacillus brevis BS6 glnR gene,and the promoter sequence containing GlnR box of glnRA,amtB-glnK and glnPQ operons were obtained by PCR.By adopting the method of bacterial one hybrid,the results proved the combination between GlnR and the promoter of the three operons in vivo,which indicates GlnR plays a significant role in nitrogen regulation of L.brevis.

关 键 词：短乳杆菌 GlnR 细菌单杂交 氮代谢调控 

分 类 号：TS201.3[轻工技术与工程—食品科学]