羊肚菌菌丝体液体培养富锌条件的优化  

Optimization of the conditions of Morchella esculenta mycelium liquid culture Zinc-riching

作　　者：王艳[1] 冮洁[1] 金李玲[1] 沈冰[1] 田朝杰[1] 王婷[1] 闫卉新[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600

出　　处：《食品工业科技》2013年第3期281-283,287,共4页Science and Technology of Food Industry

基　　金：2012年度国家民委科研项目;大学生创新创业训练计划项目(G2012001);大连民族学院自主基金(财政专项-中央高校基本科研业务费)项目(DC10020106)

摘　　要：通过对蛹虫草(Cordyceps militaris)、大球盖菇(Stropharia rugoso-annulata)、铆钉菇(Gomphidiaceae)、杏鲍菇(Pleurotus eryngii)、美味牛肝菌(Boletus edulis)、羊肚菌(Morehella esculenta)、灵芝(Ganoderma lucidum)和真姬菇(Hypsizygus marmoreus)等食用菌富锌能力的考察,筛选出具有较强的耐锌和富锌能力的羊肚菌做为富锌菌种,并采用液体发酵技术对羊肚菌菌丝体富锌适宜的锌源、锌浓度和发酵条件进行了优化。结果表明:羊肚菌菌丝体富锌适宜的锌源是Zn(CH3COO)2,在100～800mg/L的锌浓度范围内菌丝体都可以生长,菌丝体对锌的最适富集浓度为600mg/L。羊肚菌菌丝体最优富锌条件为:发酵温度25℃,起始pH7,振荡转速150r/min,250mL三角瓶装液50mL,锌添加量为600mg/L,接种量5%(V∶V)培养时间4d时,羊肚菌菌丝体的生物量及含锌量达最高,总富锌率可达23.20%,富集锌的有机化程度为37.71%。The abilities of Zinc-riching of Cordyceps militaris,Stropharia rug-annulata,Gomphidiaceae,Pleurotus eryngii,Boletus edulis,Morehella esculenta,Ganoderma lucidum and Hypsizygus marmoreus were studied. The mycelium liquid fermentation conditions of Zinc-riching of Morchella esculenta were studied. The results showed that Morchella esculenta mycelium had high capacities of Zn-resisting and Zn-accumulating. The optimal Zinc source was Zn（ CH 3 COO） 2 . The strain could grow with Zinc concentration from 100mg /L to 800mg /L. When Zinc added to the liquid medium 600mg /L,the mycelium had the most Zinc absorbance.The optimum Zinc-riching liquid fermentation conditions were as following：fermention temperature 25℃,pH7,stirring speed 150r /min,250mL shake flash containing 50mL medium,adding Zinc 600mg /L,inoculation size 5% and culture for 4d.Under this condition,the ratio of mycelia Zinc-riching reached a maximum of 23.20%,and the degree of Zinc organic was 37.71%.

关 键 词：羊肚菌 富集锌 液体发酵 菌丝体 

分 类 号：TS201.4[轻工技术与工程—食品科学]