液相色谱-串联质谱测定食用植物油和调味油中27种添加剂(物)  

Simultaneous Determination of 27 Food Additives in Vegetable Oil and Seasoning Oil by High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：曹娅[1,2] 孙利[1] 凌云[1] 赵延胜[1,3] 高慧[1,2] 冯峰[1] 储晓刚[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]山东农业大学食品科学与工程学院,山东泰安271018 [3]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品科学》2013年第4期129-134,共6页Food Science

基　　金："十一五"国家科技支撑计划项目(2009BADB9B02);国家质检总局科技计划项目(2010IK138);北京市科技计划项目(D08050200310803)

摘　　要：建立食用植物油和调味油中18种食品添加剂和9种非法添加物的液相色谱-串联质谱分析方法。样品经正己烷稀释后,采用乙腈振荡提取,乙腈层定容后直接测定18种添加剂;正己烷层浓缩后采用凝胶渗透色谱除去油脂,洗脱液浓缩后进行9种非法添加物的分析。在电喷雾电离模式下采用多反应监测模式进行测定,27种添加剂(物)在各自质量浓度范围内线性良好,线性相关系数大于0.983。空白样品添加的回收率在78.2%～108.3%之间;精密度为0.9%～17.7%。将该方法应用于市售样品的检测,发现5个样品中含有抗氧化剂TBHQ,但是均低于我国限量要求。A high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） method for simulataneous determination of 18 food additives and 9 illegal additives in vegetable oil and seasoning oil was developed. Samples were diluted with hexane prior to extraction with acetonitrile by shaking. The acetonitrile layer was diluted to a constant volume and analyzed for 18 food additives. For the analysis of 9 illegal additives, the hexane layer was concentrated and degreased by gel permeation chromatography （GPC）. Mass spectral data were acquired in electrospray ion （ESI） mode using multiple reaction monitoring （MRM） scan function. An excellent linear relationship in their respective concentration ranges was observed for all the analytes with a correlation coefficient larger than 0.983. The average recoveries from spiked blank samples ranged from 78.2% to 108.3% with relative standard deviation between 0.9% and 17.7%. TBHQ levels below the national maximum limit were detected in five commercial samples by the HPLC-MS/MS method.

关 键 词：食用植物油 食用调味油 食品添加剂 非法添加物 液相色谱-串联四极杆质谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]