喷雾干燥法制备柚苷酶固体酶制剂的工艺优化  

Technology Research for Producing Solid Naringinase Preparation by Spray Drying

作　　者：肖安风[1,2,3] 丁涛[1] 倪辉[1,2,3] 李利君[1,2,3] 杨远帆[1,2,3] 蔡慧农[1,2,3] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]福建省高校食品微生物与酶工程研究中心,福建厦门361021 [3]厦门市食品与生物工程技术研究中心,福建厦门361021

出　　处：《中国食品学报》2013年第3期92-100,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：福建省科技计划重点项目(No.2011N1008);福建省发改委产业技术开发专项项目(201011040004);厦门市科技计划项目(3502Z20093023)

摘　　要：以黑曲霉DB056为柚苷酶产生菌,柚苷酶发酵液经冷冻离心、除菌过滤、超滤浓缩和喷雾干燥得到固体酶制剂。以α-L-鼠李糖苷酶和柚苷酶酶活回收率为指标,在单因素试验的基础上,采用响应面分析法优化喷雾干燥参数,得到最佳喷雾干燥条件是:以麦芽糊精为助干剂,调整可溶性固形物含量为27%,进风温度111.3℃,进料速度161.5mL/h,热风流量5.1m^3/min,出风温度60℃,雾化器压力0.4MPa。在此条件下,α-L-鼠李糖苷酶酶活回收率为79.7%。采用优化的工艺制备柚苷酶固体酶制剂,所得酶制剂中α-L-鼠李糖苷酶活力达19000U/g,酶活总回收率为78.1%;柚苷酶的活力4700U/g,酶活总回收率为28.3%;酶制剂含水量6.5%。In this paper, solid naringinase preparation was obtained by the process of freezing centrifugation, aseptic filtration, uhrafihration enrichment and spray drying. To enhance enzyme activity recovery of ct-L-rhamnosidase and naringinase, spray drying technology was optimized by means of single factor experiment and response surface analysis. The best spray drying condition was as follows： mahodextrin was selected as drying aid, soluble solids content 27%, in- let air temperature 111.3 ℃, feeding speed 161.5 mL/h, hot air flow rate 5.1 m3/min, outlet air temperature 60 ℃, and atomizer pressure 0.4 MPa. Under this condition, the activity recovery of α-L-rhamnosidase could reach 79.7%. Using the established craft steps and parameters, solid naringinase preparation with 6.5% water content was made, and its activity and total activity recovery of α-L-rhamnosidase reached 19 000 U/g and 78.1%, as well as the activity and total activity recovery of naringinase were 4 700 U/g and 28.3%, respectively.

关 键 词：喷雾干燥 柚苷酶 固体酶制剂 优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]