表没食子儿茶素没食子酸酯与猪胰脂肪酶的相互作用  

Interaction of Epigallocatechin-3-gallate with Porcine Pancreas Lipase

作　　者：范志飞[1] 曾维才[1] 戴吉领[1] 何强[1] 

机构地区：[1]四川大学轻纺与食品学院,四川成都610065

出　　处：《食品科学》2013年第7期20-23,共4页Food Science

基　　金：国家自然科学基金项目(NSFC-31171656)

摘　　要：通过荧光光谱法研究表没食子儿茶素没食子酸酯(EGCG)与猪胰脂肪酶(PPL)的相互作用,并基于分子对接技术对其作用机理进行探讨。结果表明:EGCG对PPL具有较强的荧光猝灭作用,并抑制其催化活性,而金属离子(Ca2+、Mg2+、Cu2+、Fe2+)能减弱EGCG对PPL活性的抑制。分子对接研究表明,EGCG可通过疏水键、氢键及Pi-Pi堆积作用与PPL结合,阻碍底物进入酶活中心,从而抑制其活性。The interaction between epigallocatechin-3-gallate （EGCG） and porcine pancreas lipase （PPL） was studied by fluorescence spectroscopy, and the mechanism was investigated according to molecular docking. Results showed that EGCG could quench PPL fluorescence and inhibit its catalytic activity, while the inhibition was weakened in the presence of Ca^2＋, Mg^2＋, Cu^2＋ or Fe^2＋. Molecular docking suggested that EGCG might be bound to PPL through hydrophobicity, hydrogen bonds and Pi-Pi interaction, and blocked the entry of substrate into the active center, thus inhibiting the catalytic activity of PPL.

关 键 词：表没食子儿茶素没食子酸酯 猪胰脂肪酶 荧光猝灭 分子对接 

分 类 号：TS201.2[轻工技术与工程—食品科学]