一株解淀粉芽孢杆菌产糖条件的优化  

Optimization of Fermentation Conditions for Exopolysaccharide Production by Bacillus amyloliquefaciens LPL061

作　　者：李彦岩[1] 张彩[1] 范熠[1] 刘丽莎[1] 李平兰[1] 韩玉竹[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2013年第7期185-189,共5页Food Science

基　　金：全国大学生创新创业项目(201210019088);北京市自然科学基金项目(5122018)

摘　　要：为提高一株解淀粉芽孢杆菌LPL061胞外多糖(EPS)产量,采用单因素试验和响应面法,对该菌株的最适培养基成分和发酵条件进行优化。优化后培养基配方为:蔗糖22g/L、酵母膏18.4g/L、pH 7.0;发酵条件为:温度28℃、转速220r/min、接种量3%、发酵时间24h。优化后胞外多糖产量达4.46g/L,比优化前提高了1.51倍。In order to improve expolysaccharide （EPS） production by Bacillus amyloliquefaciens LPL061, the medium and fermentation conditions were optimized by one-factor-at-a-time design and response surface analysis. The optimal culture medium consisted of 22 g/L sucrose and 18.4 g/L yeast extract at pH 7.0. The optimal fermentation conditions were 28 ℃, 220 r/min shaking speed, 3% inoculum size and 24 h. Under these conditions, the EPS yield was 4.46 g/L, 1.51-fold higher than before the optimization.

关 键 词：解淀粉芽孢杆菌 胞外多糖 条件优化 响应面分析法 

分 类 号：TS201.3[轻工技术与工程—食品科学]