Folin-Ciocalteu法测定苹果醋饮料的总多酚含量  

Determination of Total Polyphenols in Apple Vinegar Drink by Folin-Ciocalteu Method

作　　者：赵晓娟[1] 李敏仪[1] 黄桂颖[1] 陈悦娇[1] 

机构地区：[1]仲恺农业工程学院轻工食品学院,广东广州510225

出　　处：《食品科学》2013年第8期31-35,共5页Food Science

基　　金：国家自然科学基金青年科学基金项目(21005091);仲恺农业工程学院科研基金项目(G3100021)

摘　　要：研究Folin-Ciocalteu法测定苹果醋饮料中总多酚含量的最适测定条件,并对该方法进行了分析评价。结果表明多酚含量的最佳测定条件为:样品用量1.0mL、福林-酚试剂2.5mL、12%碳酸钠溶液4.0mL、反应温度45℃、反应时间1.5h、测定波长756nm。吸光度与多酚含量在0～6μg/mL范围内呈良好的线性关系(y=0.1238x+0.0098,R2=0.9992),方法的加标回收率为101.2%,相对标准偏差为2.8%(n=5)。该方法精密度高、稳定性好,适用于苹果醋饮料中总多酚含量的测定。The optimum condition on determining total polyphenols content in apple vinegar drink by Folin-Ciocalteu method was studied. The results indicated that the optimum conditions for total polyphenols determination by Folin- Ciocalteu were as follows： 2.5 mL of Folin-phenol reagent was added in 1.0 mL of sample, and then 4.0 mL of 12% sodium carbonate solution was added. After the coloration reaction was for 1.5 h at 45 ℃, the optical density was measured at 756 nm. Under the optimum conditions, the absorbance increased linearly with the concentration of gallic acid in the range of 0 to 6 pg/mL （y=0.1238x＋0.0098, R2=0.9992）. The average spiked recovery rate was 101.2% with the relative standard deviations of 2.8% （n=5）. The method is highly precise and stable, and suitable for determining total polyphenol content in apple vinegar drink.

关 键 词：Folin—Ciocalteu 分光光度法 苹果醋 多酚 

分 类 号：TS207.3[轻工技术与工程—食品科学]