高效液相色谱法测定山楂中芦丁、金丝桃苷和槲皮素的含量  

Determination of rutin,hyperoside and quercetin in extract from Crataegus pinnatifida Bge.by HPLC

作　　者：王珍[1] 李秀霞[1] 李娇[1] 励建荣[1] 

机构地区：[1]渤海大学化学化工与食品安全学院、辽宁省食品安全重点实验室、辽宁省高校重大科技平台“食品贮藏加工及质量安全控制工程技术研究中心”,辽宁锦州121013

出　　处：《食品工业科技》2013年第9期302-305,共4页Science and Technology of Food Industry

基　　金：“十二五”国家科技支撑计划(2012BAD29B06);辽宁省食品安全重点实验室开放课题(LNSAKF20110XX)

摘　　要：旨在建立HPLC-DAD测定山楂总黄酮提取物中芦丁、金丝桃苷和槲皮素含量的方法。以70%乙醇为提取溶剂,采用Develosil C30色谱柱(250mm×4.6mm,5μm)分离,以乙腈和0.4%磷酸水溶液为流动相,流速1.0mL/min,检测波长360nm,采用峰面积和保留时间对3种黄酮进行定性定量分析。结果表明:芦丁、金丝桃苷和槲皮素这3种黄酮在30min内较好分离,其质量浓度分别为0.0050～0.1600mg/mL、0.0016～0.0500mg/mL和0.0005～0.0150mg/mL时与峰面积线性关系良好,相关系数分别为0.9999、0.9996和0.9997;该检测方法精密度的相对标准偏差(RSD)为0.48%～0.78%,回收率为92.36%～98.64%,说明该HPLC检测方法适用于山楂果粉等干植物原料中芦丁等3种黄酮类化合物的精确定量。A gradient high performance liquid chromatography （HPLC）coupled with UV-vis photodiode array detection（DAD） was developed for the separation of rutin, hyperoside, and quercetin from the flavoind extracts from hawthorn powder. The flavoinds were extracted using the solvent of 70% ethanol （by volume）. Chromatographic separation was performed in DevelosilC30 column （250mm ×4.6ram ID, 5μm）with an eluent consisting of acetonitrile and 0.4% Phosphate at a flow rate of 1.0mL/min,the eluate was monitored at 360nm.The result showed that separation and identification of rutin, hyperoside, quercetin, and other components were completed in 30min.The linear ranges of rutin, hyperoside,and quercetin were 0.0050-O.1600mg/mL（ R2 =0.9999）, 0.OO16-0.0500mg/mL（ R2 = 0.9996） and 0.0005 -0.0150mg/mL （ R2 = 0.999？ ）, respectively.This method was precise and accurate（ RSD 0.48%-0.78% ）, the average recoveries ranged from 92.36% to 98.64%, and can be used for the precise quantification of the three fiavoinds and other fiavoinds of dry plant material such as hawthorn power.

关 键 词：山楂 金丝桃苷 芦丁 槲皮素 高效液相 

分 类 号：TS207.3[轻工技术与工程—食品科学]