实时荧光定量PCR技术在金黄色葡萄球菌检测中的应用  

Application of Real-time PCR technology in Staphylococcus aureus detection

作　　者：韩齐[1] 孔保华[1] 李沛军[1] 李暮春[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2013年第9期359-363,共5页Science and Technology of Food Industry

基　　金：国家十二五科技支撑计划(2012BAD28B02)

摘　　要：金黄色葡萄球菌(Staphylococcus aureus)是主要的食源性致病菌之一。实时荧光定量PCR(Real-time PCR)技术是近年来广泛应用于食源性致病菌的快速检测方法,具有特异性强、灵敏度高等特点。本文综述了Real-time PCR技术原理、分类及其在检测食品中金黄色葡萄球菌的应用,并对其应用前景进行了展望。Staphylococcus aureus is one of the most important food- borne pathogens. Recently, Real-time Flurescent Quantitive Polymerase Chain Reaction（Real-time PCR）technology as a modern method was exploited to detect food- borne pathogens for its specific and sensitive attributes. In this paper, the principle and classification of Real-time PCR technology was introduced briefly,and its application in detecting Staphylococcus aureus was reviewed.Also,the application of this technique in S.aureus detection was prospected.

关 键 词：食品 金黄色葡萄球菌 实时荧光定量PCR . 检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]