液体发酵生产古尼虫草多糖的营养条件优化  

Optimization of nutritional conditions for promotion of polysaccharide produced by Cordyceps gunnii in submerged culture

作　　者：刘晓翠[1] 朱振元[1] 孙会轻[1] 李园园[1] 代丽翠[1] 张静怡[1] 吕茜[1] 高辉[1] 

机构地区：[1]食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品工业科技》2013年第10期225-229,共5页Science and Technology of Food Industry

基　　金：国家农业科技成果转化资金项目(2011GB2A100009);国家高新技术研究计划(863计划)项目(2012AA021505);天津市高等学校科技发展基金计划项目(20090604);天津科技大学科学研究基金资助项目(20120106)

摘　　要：以提高古尼虫草菌丝体多糖的产量为目的,对古尼虫草液体发酵的营养条件进行优化。以三角瓶摇床培养中胞内多糖产量作为优化指标,对培养基中碳源、氮源的种类及添加量进行了筛选。碳源单因素实验结果表明最佳碳源为蔗糖,添加量为4%,此时胞内多糖产量高达147.40mg/100mL;氮源单因素实验结果显示蛋白胨为最佳氮源,添加量为1.0%,此时胞内多糖产量高达102.49mg/100mL。正交实验优化后营养条件为:蔗糖4%,蛋白胨1.0%,KH_2PO_4 0.05%,MgSO_4·7H_2O 0.1%,在此条件下,多糖产量可达(181.5±2.3)mg/100mL。In order to improve the polysaccharide production of Cordyceps gunnii,the optimization of the nutritional conditions of the liquid fermentation was carried out. With the production of intracellular polysaccharide （IPS） which was shaking cultivated in triangular bottles as optimization indexes to select carbon source types, nitrogen source types and add quantity of them. Results of carbon source single factor experimental showed that the best carbon source was sucrose,and when the adding amount was 4% ,the yield of IPS up to 147.40mg/100mL. Results of nitrogen source single factor experimental showed that the best nitrogen source was peptone,and when the adding amount was 1.0%,the yield of IPS up to 102.49mg/100mL. The results of the orthogonal test indicated that the optimum nutritional condition was sucrose 4% ,peptone 1.0% ,KH2PO4 0.05% and MgSO4 7H20 0.1%. Under this condition,the polysaccharide production reached （181.5＋2.3）mg/ 100m L.

关 键 词：古尼虫草 多糖 液体发酵 营养条件 

分 类 号：TS201.2[轻工技术与工程—食品科学]