不同方法对大米中无机砷含量测定结果的影响  

Influence of determination methods on the content of inorganic arsenic in rice

作　　者：岳兵[1] 刘丽萍[2] 谢科[1] 潘浩[1] 尚晓虹[1] 李筱薇[1] 赵云峰[1] 吴永宁[1] 

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021 [2]北京市疾病预防控制中心中心实验室,北京100013

出　　处：《中国食品卫生杂志》2013年第3期238-240,共3页Chinese Journal of Food Hygiene

基　　金："十二五"国家科技支撑计划(2012BAK01B01)

摘　　要：目的建立不同方法测定大米和以大米为原料的食品中无机砷的含量,并对结果进行比较。方法用0.15 mol/L硝酸或0.3 mol/L三氟乙酸作为提取液,95℃下热辅助提取2 h、75℃下超声提取2 h或90℃下微波辅助提取1 h,提取物经阴离子交换柱X-100(250 mm×4.1 mm,10μm)分离,电感耦合等离子体-质谱(ICP-MS)和氢化物发生-原子荧光光谱(HG-AFS)分别测定。结果两种方法测定无机砷含量,加标回收率分别在94.8%～111.7%、91.9%～96.0%之间,相对标准偏差均小于5.8%,应用这两种方法对质控样品米粉SRM 1568a和大米样品进行测定,结果协同性良好。结论两种方法均可简单、准确、快速的测定大米中的无机砷,结果比较差异无统计学意义。Objective To establish methods for the detection of inorganic arsenic in rice and rice-based foodstuff and compare the results deviation of various extracting and/or instrumental process. Methods The extraction conditions for inorganic arsenic extraction in rice were O. 15 tool/L nitric acid or 0. 3 mol/L TFA ; heat-assistant extraction 2 h, 95℃ ; ultrasonic 2 h, 75℃ ; microwave-assist 1 h , 90℃. Then the extracts were separated by an anion exchange column Hamilton PRP X-IO0 （250 mm×4. 1 mm, 1μm） and detected by ICP-MS and HG-AFS respectively. Results The determination of inorganic arsenic of these two methods was good. The recoveries of spiked samples were 94. 8% - 111.7% and 91.9% -96.0% , and the relative standard deviations （RSD, n =6） were below 5.8%. The results were satisfying for control sample （SRM1568a） and rice samples. Conclusion The methods were simple, accurate and fast for the determination of inorganic arsenic in rice and rice-based foodstuff. There was no significant statistic difference among the results.

关 键 词：无机砷 大米 电感耦合等离子体-质谱 氢化物发生-原子荧光光谱 

分 类 号：R155.5[医药卫生—营养与食品卫生学]