刺五加苷食用安全性毒理学评价  

Food Safety and Toxicological Assessment of Eleutheroside

作　　者：杨平[1] 李润国[1] 孟宪军[2] 

机构地区：[1]沈阳师范大学粮食学院,辽宁沈阳110034 [2]沈阳农业大学食品学院,辽宁沈阳110161

出　　处：《食品科学》2013年第11期258-262,共5页Food Science

基　　金：辽宁省自然科学基金项目(2011205001)

摘　　要：目的:研究刺五加苷的食用安全性,为促进其合理开发利用提供科学依据。方法:根据《保健食品检验与评价技术规范》,对刺五加苷进行急性毒性实验、小鼠骨髓微核实验、Ames实验、小鼠精子畸变实验、大鼠30d喂养实验。结果:刺五加苷对雌雄小鼠的急性经口的最大耐受剂量(MTD)均大于20g/kg(以体质量计);Ames实验、小鼠骨髓微核实验、小鼠精子畸形实验均为阴性;大鼠30d喂养实验中,实验动物生长情况良好,血液学检查、生化学检查、主要脏体比及组织学检查结果与对照组相比,均无明显差异。结论:刺五加苷属实际无毒物质,未显示有遗传毒性,初步估计其未观察到损害作用的剂量(NOAEL)为20g/kg。Objective： To evaluate the safety of eleutheroside, and to provide scientific support for the reasonable utilization and development of eleutheroside. Methods： According to the national standard procedures and methods in food safety and toxicological evaluation, a series of toxicological studies on the ingestion of eleutheroside were conducted, which included acute toxicity, genetic toxicity and subacute toxicity tests. Results： For acute toxicity, the maximum tolerated dose （MTD） was higher than 20 g/kg. The results of Ames test, mouse bone marrow micronucleus test and mouse sperm abnormality test were negative. After 30 d of feeding, rats showed no significant difference in body growth, organ development or histological observation. Conclusion： Eleutheroside is actually non-toxic with no genetic toxicity. No adverse effect of eleutheroside at 20 g/kg has been observed during trails for 30 d.

关 键 词：刺五加苷 急性毒性 遗传毒性 亚急性毒性 食品安全 

分 类 号：TS201.1[轻工技术与工程—食品科学]