SPME萃取头对大菱鲆鱼肉挥发性成分萃取效果分析  

Analysis of volatile compounds in Turbot by SPME with different extraction fibers

作　　者：徐永霞[1] 刘滢[1] 宋帅婷 桑敏 励建荣[1] 陈华健 

机构地区：[1]渤海大学食品科学研究院,渤海大学化学化工与食品安全学院,辽宁省食品安全重点实验室,"食品贮藏加工及质量安全控制工程研究中心"辽宁省高校重大科技平台,辽宁锦州121013 [2]湛江国联水产开发股份有限公司,广东湛江524000

出　　处：《食品工业科技》2013年第12期90-93,97,共5页Science and Technology of Food Industry

基　　金："十二五"国家科技支撑计划(2012BAD29B06);辽宁省食品安全重点实验室开放课题(LNSAKF2011023)

摘　　要：采用DVB/CAR/PDMS、PDMS/DVB和CAR/PDMS3种萃取头进行顶空-固相微萃取(HS-SPME),提取新鲜大菱鲆鱼肉的挥发性成分,结合气质联用仪(GC-MS)进行分析鉴定,确定最佳的萃取头。结果表明,3种萃取头固相微萃取法共检测出81种挥发性成分,主要是醛、酮、醇、烯烃、烷烃和胺类化合物。使用DVB/CAR/PDMS、PDMS/DVB和CAR/PDMS萃取头分别检测出51、37和29种挥发性成分,其中使用DVB/CAR/PDMS萃取头检测出的挥发性物质总峰面积最大且种类最多。因此,DVB/CAR/PDMS萃取头较适合于大菱鲆鱼肉挥发性成分的提取。The volatile compounds in turbot were extracted by headspace solid phase microextraction（HS- SPME） with PDMS/DVB,CAR/PDMS and DVB/CAR/PDMS fibers and analyzed by gas chromatography-mass spectrometry（GC-MS）. The results showed that total 81 volatile compounds were identified. Among the identified compounds, aldehydes, ketones, alcohols, alkenes, alkanes and amines were predominant. There were 51 ,37 and 29 kinds of volatiles extracted by DVB/CAR/PDMS, PDMS/DVB and CAR/PDMS fiber respectively. And the volatile compounds extracted by DVB/CAR/PDMS fiber had highest total peak areas and most varieties. Therefore,the DVB/CAR/PDMS fiber was the most suitable for extracting volatile compounds in turbot.

关 键 词：大菱鲆 挥发性成分 萃取头 顶空-固相微萃取 气相色谱-质谱法 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]