弱后酸化保加利亚乳杆菌突变株与亲本菌株H^+-ATPase基因的相似性比较  

Comparison of similarity of H^+-ATPase coding gene between wild strain and mutant strain with low post-acidification of Lactobacillus delbrueckii subsp.bulgaricus

作　　者：刘飞[1] 焦月华 郭文奎[1] 于微[1] 谷春涛[1] 霍贵成[1] 

机构地区：[1]东北农业大学,乳品科学教育部重点实验室,黑龙江哈尔滨150030 [2]黑龙江省中医药大学,药物安全性评价中心,黑龙江哈尔滨150040

出　　处：《食品工业科技》2013年第15期127-130,135,共5页Science and Technology of Food Industry

基　　金：教育部长江学者和创新团队发展计划资助(IRT0959);国家863计划项目(2011AA100902);国家自然科学基金(30972131)

摘　　要：为了确定德氏乳杆菌保加利亚亚种H+-ATPase的调控机制,以H+-ATPase缺陷的德氏乳杆菌保加利亚亚种突变菌株和亲本菌株KLDS1.9201作为研究对象。首先分别提取亲本和突变菌株的基因组DNA,随后利用PCR的方法扩增出H+-ATPase蛋白的全部编码基因并测序,最后利用DNAMAN软件比较亲本和突变菌株的H+-ATPase的相似性。结果表明亲本和突变菌株H+-ATPase编码基因的相似性为100%。H+-ATPase缺陷的德氏乳杆菌保加利亚亚种突变菌株KLDS1.9201-11和亲本菌株KLDS1.9201的H+-ATPase活力之间的差异并不是由于编码基因发生了突变,可能是转录水平的降低导致H+-ATPase酶蛋白的表达量降低,进而影响酶活力。为进一步研究德氏乳杆菌保加利亚亚种H+-ATPase的调控机制奠定了基础。In order to determine the regulation mechanism of H^＋- ATPase of Lactobacillus delbrueckii subsp. bulgaricus, a H ＋- ATPase deficiency strain Lactobacillus delbrueckii subsp, bulgaricus KLDS1.9201 - 11 with low post-acidification which was derived from Lactobacillus delbrueckii subsp, bulgaricus KLDS1.9201 in previous research and the wild type strain KLDS1.9201 were investigated.The genomic DNA was extracted from KLDS1.9201 -11 and KLDS1.9201 respectively, and the coding genes of H ＋-ATPase of these two strains were acquired by PCR amplification,then PCR products were sequenced.At last the similarity of coding genes of H^＋-ATPase between the KLDS1.9201-11 and KLDSl.9201 was compared by the software of DNAMAN.Results showed that the similarity of coding genes of H ＋-ATPase between the KLDS1.9201-11 and KLDS1.9201 was 100%.In conclusion the differential of H＋-ATPase activity of KLDS1.9201-11 and KLDSL9201 was not caused by the mutadon of coding gene of H^＋- ATPase.And it might the lower expression of H^＋-ATPase of mutant strain KLDS1.9201-11 which in turn caused the reduced H＋-ATPase activity.It provided the theoretical basis for further investigating the regulation mechanism of H ＋-ATPase of Lactobacillus delbrueckii subsp, bulgaricus.

关 键 词：德氏乳杆菌保加利亚亚种 H+-ATPase 相似性 后酸化 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]