漠斑牙鲆肌肉氨基酸和脂肪酸组成分析与营养品质评价  

Amino Acid and Fatty Acid Compositions and Nutritional Quality of Muscle in Southern Flounder, Paralichthys lethostigma

作　　者：许建和[1] 秦洁[2] 邹宽[1] 马欢迎 

机构地区：[1]淮海工学院江苏省海洋生物技术重点实验室,江苏连云港222000 [2]上海海洋大学水产与生命学院,上海215104 [3]淮安通威水产饲料有限公司,江苏淮安223005

出　　处：《食品科学》2013年第15期299-302,共4页Food Science

基　　金：江苏省自然科学基金项目(BK2009652);江苏省教育厅高校自然科学基金项目(11KJD170001);江苏省海洋生物技术重点实验室开放课题(2007HS001)

摘　　要：对人工养殖漠斑牙鲆肌肉进行一般营养组分、氨基酸和脂肪酸组成分析,并对其营养品质进行评价。结果表明:漠斑牙鲆肌肉鲜样中粗蛋白和粗脂肪含量分别为17.74%和3.02%。肌肉粗蛋白中检测出19种氨基酸,其中谷氨酸(120.4mg/g pro)、天冬氨酸(75.4mg/g pro)、甘氨酸(73.6mg/g pro)、亮氨酸(72.3mg/g pro)、赖氨酸(67.8mg/g pro)和精氨酸(63.4mg/g pro)含量较高。依据世界粮农组织/世界卫生组织/联合国大学(FAO/WHO/UNU)必需氨基酸基准模式,10种必需氨基酸评分均大于100,表明漠斑牙鲆肌肉蛋白是氨基酸平衡性较好的优质蛋白。脂肪酸检测结果显示漠斑牙鲆肌肉中饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸含量分别为32.23%、30.79%和27.01%。n-3系列多不饱和脂肪酸中二十碳五烯酸(EPA,C20:5n-3)和二十二碳六烯酸(DHA,C22:6n-3)含量较高,为7.38%和12.07%;n-6系列多不饱和脂肪酸中花生四稀酸(AA,C20:4n-6)含量最高,为4.49%。n-6和n-3系列多不饱和脂肪酸比值为0.35,远低于我国和英国卫生部推荐的4.0最高安全限值。The proximate composition and amino acid and fatty acid profiles of muscle in cultured southern flounder, Paralichthys lethostigma, were analyzed to evaluate its nutritional quality. Southern flounder muscle contained 17.74% crude protein and 3.02% crude lipid. In addition, Nineteen amino acids were detected, including a large amount of glutamic acid （120.4 nmg/g crude protein）, aspartic acid （75.4 mg/g crude protein）, glycine （73.6 mg/g crude protein）, leucine （72.3 mg/g crude protein）, lysine （67.8 mg/g crude protein） and arginine （63.4 mg/g crude protein）. According to the reference amino acid pattern recommended by FAO/WHO/UNU （1985） for pre-school children （2 to 5 years old）, the scores for all the essential amino acids were over 100 indicating the muscle of southern flounder to contain high-quality protein with a good amino acid balance. Total saturated fatty acid, monounsaturated fatty acid and polyunsaturated fatty acid （PUFA） contents in the muscle of southern flounder were 32.23%, 30.79% and 27.01%, respectively. In n-3 PUFAs, 7.38% eicosapentaenoic acid （EPA, C20：5n-3） and 12.07% docosahexaenoic acid （DHA, C22：6n-3） were detected, while 4.49% arachidonic acid （AA, C20：4n.6） was detected in n-6 PUFAs. The ratio of ∑PUFA n-61 F. PUFA n-3 was 0.35, which was significantly lower than the maximum safety value of 4.0 recommended by the UK Department of Health.

关 键 词：漠斑牙鲆 一般营养组分 脂肪酸 氨基酸 营养评价 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]