Dihydroartemisinin promotes angiogenesis during the early embryonic development of zebrafish  

作　　者：Qian BA Juan DUAN Jia-qiang TIAN Zi-liang WANG Tao CHEN Xiao-guang LI Pei-zhan CHEN Song-jie WU Li XlANG Jing-quan LI Rui-ai CHU Hui WANG 

机构地区：[1]Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences University of Chinese Academy of Sciences, Shanghai 200031, China [2]Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China

出　　处：《Acta Pharmacologica Sinica》2013年第8期1101-1107,共7页中国药理学报（英文版）

摘　　要：Aim： To investigate the embryotoxicity of dihydroartemisinin （DHA）, the main active metabolite of artemisinin, in zebrafish, and explore the corresponding mechanisms. Methods： The embryos of wild type and TG （flkl：GFP） transgenic zebrafish were exposed to DHA. Developmental phenotypes of the embryos were observed. Development of blood vessels was directly observed in living embryos of TG （flkl：GFP） transgenic zebrafish under fluorescence microscope. The expression of angiogenesis marker genes vegfa, ilk1, and fit1 in the embryos was detected using real-time PCR and RNA in situ hybridization assays. Results： Exposure to DHA （1-10 mg/L） dose-dependently caused abnormal zebrafish embryonic phenotypes in the early developmental stage. Furthermore, exposure to DHA （10 mg/L） resulted in more pronounced embryonic angiogenesis in TG （flkl：GFP） zebrafish line. Exposure to DHA （10 mg/L） significantly increased the mRNA expression of veEfa, flkl, and fit1 in the embryos. Knockdown of the ilk1 protein partially blocked the effects of DHA on embryogenesis. Conclusion： DHA causes abnormal embryonic phenotypes and promotes angiogenesis in zebrafish early embryonic development, demonstrating the potential embryotoxicity of DHA.Aim： To investigate the embryotoxicity of dihydroartemisinin （DHA）, the main active metabolite of artemisinin, in zebrafish, and explore the corresponding mechanisms. Methods： The embryos of wild type and TG （flkl：GFP） transgenic zebrafish were exposed to DHA. Developmental phenotypes of the embryos were observed. Development of blood vessels was directly observed in living embryos of TG （flkl：GFP） transgenic zebrafish under fluorescence microscope. The expression of angiogenesis marker genes vegfa, ilk1, and fit1 in the embryos was detected using real-time PCR and RNA in situ hybridization assays. Results： Exposure to DHA （1-10 mg/L） dose-dependently caused abnormal zebrafish embryonic phenotypes in the early developmental stage. Furthermore, exposure to DHA （10 mg/L） resulted in more pronounced embryonic angiogenesis in TG （flkl：GFP） zebrafish line. Exposure to DHA （10 mg/L） significantly increased the mRNA expression of veEfa, flkl, and fit1 in the embryos. Knockdown of the ilk1 protein partially blocked the effects of DHA on embryogenesis. Conclusion： DHA causes abnormal embryonic phenotypes and promotes angiogenesis in zebrafish early embryonic development, demonstrating the potential embryotoxicity of DHA.

关 键 词：ARTEMISININ DIHYDROARTEMISININ ZEBRAFISH ilk1 VEGF ANGIOGENESIS EMBRYOTOXICITY 

分 类 号：Q463[生物学—生理学] S512.1[农业科学—作物学]