超声波辅助纤维素酶法提取紫玉米芯色素工艺研究  

Study on Optimum Extraction Technology of Purple Pigment from Purple Corn Cob by Ultrasonic-enzyme Synergistic Method

作　　者：张康逸[1] 范运乾[1] 康志敏[1] 王继红[1] 侯传伟[1] 

机构地区：[1]河南省农业科学院农副产品加工研究所,河南郑州450002

出　　处：《河南农业科学》2013年第6期152-155,共4页Journal of Henan Agricultural Sciences

基　　金：河南省财政厅科技攻关项目

摘　　要：为了找出紫玉米芯色素的最佳提取工艺,以紫玉米芯色素提取液吸光度为提取得率评价指标,对超声波提取法、纤维素酶法、超声波辅助纤维素酶法的色素提取效果进行比较,并通过单因素和正交试验对提取效果最好的超声波辅助纤维素酶法的提取条件即超声波功率、温度、时间进行优化,确定其最佳工艺条件。结果表明,超声波辅助纤维素酶法提取紫玉米芯色素的最佳工艺条件为:10g/L纤维素酶溶液,在pH值5.0、温度50℃条件下酶解30min,然后在超声波功率250W、温度40℃下处理15min,紫玉米芯色素提取得率达到16.7%。A process route based on ultrasonic treatment and cellulase hydrolysis was designed for the preparation of purple pigment with purple corn cob as the raw material.Compared with the ultrasonic extraction and cellulase hydrolysis,ultrasonic-enzyme synergistic method had the highest extraction yield among the three extraction methods.The absorbance value of extracting solution was used as an appraisal index,and the optimum parameters of ultrasonic-enzyme synergistic method obtained by orthogonal experiments were： ultrasonic power 250 W,ultrasonic time 15 min,ultrasonic temperature 40 ℃ under the condition of cellulase enzyme concentration 10 g/L,pH 5.0,enzymolysis time 30 min and enzymolysis temperature 50 ℃.Under this condition,the extraction yield of purple pigment could reach about 16.7%.

关 键 词：紫玉米芯 紫色素 纤维素酶 超声波 

分 类 号：TS202.3[轻工技术与工程—食品科学]