五酶法提取芦笋老茎中可溶性膳食纤维工艺研究  

Five Enzymatic Study on the Extraction of Soluble Dietary Fiber from Old Stalk of Asparagus

作　　者：杨晓宽[1] 侍朋宝[1] 赵玉华[1] 张佳琳[1] 徐银玲[1] 常学东[1] 

机构地区：[1]河北科技师范学院食品科技学院河北秦皇岛066004

出　　处：《中国食品学报》2013年第5期24-31,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家星火计划项目(No.2010GA620001);河北省科技支撑项目(No.12227168);秦皇岛市科技支撑项目(No.2012022A006)

摘　　要：以芦笋老茎为原料,研磨成粉,过40目筛,在料液比1∶25g/mL的水溶液中依次用脂肪酶(100U/mg)、淀粉酶(30U/mg)和糖化酶(100U/mg)、蛋白酶(60U/mg)、纤维素酶(1∶1000U/mg)酶解,提取可溶性膳食纤维。分别考察酶添加量、酶解pH、酶解温度、酶解时间对酶作用效果的影响。在此基础上,通过正交试验确定的酶解最佳工艺是:脂肪酶添加量0.80%,酶解pH9.5,酶解温度35℃,酶解时间105min;淀粉酶和糖化酶添加量分别为0.75%和0.20%,酶解pH6.0,酶解温度70℃,酶解时间180min;蛋白酶添加量1.75%,酶解pH7.3,酶解温度43℃,酶解时间120min;纤维素酶添加量0.30%,酶解pH4.8,酶解温度60℃,酶解时间60min。可溶性膳食纤维得率13.7%,纯度高达76.45%。Old stalk of asparagus as raw materials, processing 40 mesh screening treatment of ground into a powder. In 1∶25 g/mL ratio of liquid/solid to solvent aqueous environment, using lipase in turn（100 U/mg）, amylase（30 U/mg） and Glucoamylase （100U/mg）, protease （60U/mg）, cellulose （1∶1000U/mg） by enzymatic hydrolysis and extraction of soluble dietary fiber. It is studied respectively for the enzyme amount, enzymatic hydrolysis-pH, enzymatic hydrolysis-temperature and enzymatic hydrolysis-time affecting these enzymes effect. On the basis of orthogonal test to determine the optimal conditions for enzymatic hydrolysis： lipase is added 0.8%, pH 9.5 by enzymatic hydrolysis and enzymatic hydrolysis temperature 35 ℃, enzymatic hydrolysis time 105min; Amylase and Glucoamylase are added 0.75% and 0.20%, respectively, pH 6.0 enzyme solutions, enzymatic hydrolysis temperature 70 ℃ , enzymatic hydrolysis time 180 min; Protease is added 1.75%, pH 7.3 by enzymatic hydrolysis and enzymatic hydrolysis temperature 43 ℃, enzymatic hydrolysis time 120 min; Cellulase amounts to 0.3% , enzymatic hydrolysis of pH 4.8, enzymatic hydrolysis of temperatures of 60 ℃ , enzymatic hydrolysis time 60 min. Soluble dietary fibre yield of 13.7%, high purity of 85.05%.

关 键 词：芦笋 膳食纤维 工艺优化 纤维素酶 正交试验 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]