单宁酶固体酶制剂的喷雾干燥制备工艺优化  

Technology Research for Producing Solid Tannase Preparation by Spray Drying

作　　者：肖安风[1,2,3,4] 王芳[1] 倪辉[1,2,3,4] 李利君[1,2,3,4] 杨远帆[1,2,3,4] 蔡慧农[1,2,3,4] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021 [3]福建省高校食品微生物与酶工程技术研究中心,福建厦门361021 [4]厦门市食品与生物工程技术研究中心,福建厦门361021

出　　处：《中国食品学报》2013年第7期144-151,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：福建省科技计划重点项目(2011N1008)

摘　　要：以黑曲霉JMU-TS528为单宁酶产生菌,利用茶叶梗固态发酵生产单宁酶。以单宁酶酶活回收率为评价指标,在单因素试验的基础上,结合响应面分析法对单宁酶固体酶制剂的喷雾干燥制备工艺进行优化,得到最佳喷雾干燥条件:助干剂为麦芽糊精,进风温度96℃,进料流量182 mL/h,可溶性固形物含量8%。在此条件下,单宁酶固体酶制剂的酶活力达到690 U/g,单宁酶酶活回收率达到79.8%。Took the recovery of tannase activity as the evaluation index, the optimum condmons ot spray urymg mr tannase solid enzyme preparation were determined with single factor test and response surface analysis method. The optimized spray drying parameters was shown as follows： mahodextrin used as drying aid inlet, air temperature 96 ℃, inlet flow rate 182 mL/h, soluble solids content 8%. Under the optimum conditions, the enzyme activity of tannase solid enzyme preparation reached 690 U/g, and the enzyme activity recovery was 79.8% at the same time.

关 键 词：单宁酶 固体酶制剂 喷雾干燥 工艺优化 

分 类 号：TS201.25[轻工技术与工程—食品科学]