富硒玉米醒酒肽的制备及活性研究  

Study on preparation and activity of the selenium-enriched corn peptides facilitating alcohol metabolism

作　　者：王真真[1] 何慧[1] 王驰[1] 侯焘[1] 张岩[1] 姚余祥[1] 赵宁宁[1] 

机构地区：[1]华中农业大学食品科学技术学院,环境食品学教育部重点实验室,湖北武汉430070

出　　处：《食品工业科技》2013年第18期239-242,248,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(30972043);国家"863"计划项目(2008AA10Z314)

摘　　要：为了利用硒和玉米肽的双重活性,制备出活性优于普通玉米的醒酒肽,从而提高富硒玉米的附加值,以富硒玉米为原料,从中提取蛋白质,进而制备富硒玉米肽,以其对·OH抑制率、硒回收率和肽回收率等为指标,通过单因素实验以及正交实验对酶解条件进行了优化;通过动物实验比较了两种玉米肽的醒酒活性。结果显示最优酶解条件为:料液比1∶35(w/v),温度60℃,加酶量0.6%,此时硒回收率为81.78%,肽回收率为73.32%,水解度为10.50%,酶解物对·OH的半抑制率浓度(IC50)为3.57mg/mL;200mg/kg剂量的富硒玉米肽降低小鼠血清中乙醇浓度(blood alcohol concentration,BAC)的能力极显著优于同剂量的普通玉米肽(p<0.01),400mg/kg剂量的富硒玉米肽的血醇清除率可达50.99%。富硒玉米肽的醒酒活性显著优于普通玉米肽。In order to get the higher activities selenium-enriched corn peptides with facilitating alcohol metabolism than that of corn peptides,the enzymatic hydrolysis of selenium-riched corn protein was studied. The enzymatic hydrolysis was optimized by single factor experiments and orthogonal test,the recovery of selenium/ peptides and the scavenging activity toward hydroxyl radical （·OH） as the indexes. The facilitating alcohol metabolism activity was studied by animal experiment. The optimal conditions were determined as follows ：the material to solvent ratio of 1：35（w/v）,the temperature of 60℃,the Alcalase dosage of 0.6%. Under these conditions,selenium recovery rate was as high as 81.78% ,the peptide recovery was 73.32% ,the degree of hydrolysis was 10.50% and the scavenging activity toward .OH with an IC50 value was 3.57mg/mL. The BAC was most significantly reduced in selenium-enriched corn peptides group at the dose of 200mg/kg doses when compaird to the corn peptides group with the same dose（p〈0.01 ）. The selenium-enriched corn peptides at the dose of 400mg/kg could reduce 50.99% of BAC. The facilitating alcohol metabolism activity of selenium- enriched corn peptides was higher than corn peptides.

关 键 词：富硒玉米肽 酶解 醒酒 

分 类 号：TS201.4[轻工技术与工程—食品科学]